A molecular strategy to optimize bovine tuberculosis post-mortem diagnosis and the exposure to Mycobacterium tuberculosi
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SHORT COMMUNICATION
A molecular strategy to optimize bovine tuberculosis post‑mortem diagnosis and the exposure to Mycobacterium tuberculosis variant bovis Bruna Correa Lopes1,2 · Emily Marques dos Reis1 · Fernanda Bastos Rubin de Bitencourt1 · Márcia Regina Loiko1,3 · André Vinícius Andrade Bezerra1 · Thais Silveira Bueno1 · Isadora Tadeval Lape1,5 · Cristine Cerva1 · Mário de Menezes Coppola1 · Rogério Oliveira Rodrigues1 · José Eduardo Vargas4 · Angélica Cavalheiro Bertagnolli1 · Fabiana Quoos Mayer1 Received: 6 May 2020 / Accepted: 2 August 2020 © Springer Nature B.V. 2020
Abstract Bovine tuberculosis (bTB) is a zoonotic disease caused by Mycobacterium tuberculosis var. bovis, for which the definitive diagnosis is accomplished by bacterial isolation, which has biosafety issues and requires long time. Thus, diagnostic methods with potential to be faster and more efficient can represent an advance in bTB epidemiological knowledge and decrease exposure to M. tuberculosis var. bovis. This study aimed to validate a molecular test for bTB post-mortem diagnosis, as a strategy to reduce waste in bovine production. A total of 185 tissues from animals of infected herds or with suspected lesions at abattoir were evaluated through bacterial isolation, PCR and histopathology. PCR and histopathology showed sensitivities of 45.1% and 71.2%, respectively, and specificities of 83.3% and 83.0%, respectively, when compared to bacterial isolation. The combination of both tests resulted in enhanced specificity and positive predictive values.Therefore, PCR in conjunction with histopathology may be used as screening, in which concordant results can be considered conclusive, and discordant results may be submitted to bacterial isolation. Keywords Tuberculosis · PCR · Histopathology · Cattle · Bacteriology · Diagnostic techniques
Introduction
Bruna Correa Lopes, Emily Marques dos Reis and Fernanda Bastos Rubin de Bitencourt contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11033-020-05718-7) contains supplementary material, which is available to authorized users. * Fabiana Quoos Mayer [email protected] 1
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Centro de Pesquisa em Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Departamento de Diagnóstico e Pesquisa Agropecuária, Secretaria de Agricultura, Pecuária e Desenvolvimento Rural, Eldorado do Sul, Rio Grande do Sul, Brazil Programa de Pós‑Graduação em Ciências Veterinárias, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil
Bovine tuberculosis (bTB) is an infectious disease caused by recently reclassified Mycobacterium tuberculosis var. bovis [1] that affects several species of animals, has global distribution [2, 3] and is considered re-emergent and neglected zoonotic disease by the World Health Organization (WHO). In Brazil, there is a lack of recent data on bTB prevalence, with the last official survey pointing 3
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