A quick and efficient hydroponic potato infection method for evaluating potato resistance and Ralstonia solanacearum vir
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Plant Methods Open Access
RESEARCH
A quick and efficient hydroponic potato infection method for evaluating potato resistance and Ralstonia solanacearum virulence Huijuan Wang1†, Jinxue Hu1†, Yao Lu1†, Mancang Zhang1†, Ning Qin1, Ruize Zhang1, Yizhe He1, Dongdong Wang1, Yue Chen1, Cuizhu Zhao1, Núria S. Coll2, Marc Valls2,3, Qin Chen4* and Haibin Lu1*
Abstract Background: Potato, the third most important crop worldwide, plays a critical role in human food security. Brown rot, one of the most destructive potato diseases caused by Ralstonia solanacearum, results in huge economic losses every year. A quick, stable, low cost and high throughout method is required to meet the demands of identification of germplasm resistance to bacterial wilt in potato breeding programs. Results: Here we present a novel R. solanacearum hydroponic infection assay on potato plants grown in vitro. Through testing wilt symptom appearance and bacterial colonization in aerial part of plants, we found that the optimum conditions for in vitro potato infection were using an OD600 0.01 bacterial solution suspended with tap water for infection, broken potato roots and an open container. Infection using R. solanacearum strains with differential degree of aggressivity demonstrated that this infection system is equally efficient as soil-drench inoculation for assessment of R. solanacearum virulence on potato. A small-scale assessment of 32 potato germplasms identified three varieties highly resistant to the pathogen, which indicates this infection system is a useful method for high-throughout screening of potato germplasm for resistance. Furthermore, we demonstrate the utility of a strain carrying luminescence to easily quantify bacterial colonization and the detection of latent infections in hydroponic conditions, which can be efficiently used in potato breeding programs. Conclusions: We have established a quick and efficient in vitro potato infection system, which may facilitate breeding for new potato cultivars with high resistance to R. solanacearum. Keywords: Ralstonia solanacearum, Potato, In vitro infection, Brown rot, Bacterial wilt Background Ralstonia solanacearum is the causal agent of bacterial wilt (also known as brown rot on potato), one of the most destructive plant diseases on many crops in tropical and subtropical areas, which leads to huge losses in food *Correspondence: [email protected]; luhaibin011@hotmail. com † Huijuan Wang, Jinxue Hu, Yao Lu and Mancang Zhang contributed equally to this work 1 College of Agronomy and State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China 4 State Key Laboratory of Crop Stress Biology for Arid Areas, College of Food Science and Engineering, Northwest A & F University, Yangling 712100, Shaanxi, China Full list of author information is available at the end of the article
production [1]. This soil-borne bacterium enters root through wounds or natural openings and multiplies in the vascular tissues, which results in xyl
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