Bacillus thermoamylovorans -Related Strain Isolated from High Temperature Sites as Potential Producers of Medium-Chain-L
- PDF / 1,002,491 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 31 Downloads / 181 Views
Bacillus thermoamylovorans‑Related Strain Isolated from High Temperature Sites as Potential Producers of Medium‑Chain‑Length Polyhydroxyalkanoate (mcl‑PHA) Aophat Choonut1 · Poonsuk Prasertsan2 · Sappasith Klomklao3 · Kanokphorn Sangkharak4 Received: 6 February 2020 / Accepted: 6 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Thermotolerant bacteria producing medium-chain-length polyhydroxyalkanoate (mcl-PHA) were isolated from various thermal sites, including palm oil mill effluent, textile wastewater, and hot spring water, in Thailand. Fifteen strains were isolated at 45 °C using nutrient-rich (NR) medium. However, only six isolates produced mcl-PHA at 0.41 ± 0.01 g/L to 0.80 ± 0.01 g/L, representing a mcl-PHA content of 29.44% to 50.77% of the dry cell weight (DCW). The six strains of bacterial isolates could utilise a variety of substrates; all were identified as Bacillus thermoamylovorans. The highest mcl-PHA content (50.77% of the DCW) was accumulated by the B. thermoamylovorans strain PHA005 isolated from palm oil mill effluent. The mcl-PHA from strain PHA005 was composed of five different monomers, 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxytetradecanoate (3HTD), 3-hydroxyhexadecanoic acid (3HHD), and 3-hydroxyoctadecanoic (3HOD), with a monomer content of 24.12, 15.50, 13.00, 39.25, and 8.13 mol%, respectively. The optimum temperature for B. thermoamylovorans strain PHA005 growth is 45 °C, and it can survive at up to 60 °C. This is a first report of PHA synthesis by a thermotolerant B. thermoamylovorans. Moreover, the high content of 3HHD monomers (39.25 mol%) has never been reported in Bacillus.
Introduction Polyhydroxyalkanoates (PHAs) are bio-polyesters synthesized by bacteria as intracellular storage reserves of carbon and energy. PHAs have attracted great interest due to the similarities between their material properties and the material properties of thermoplastics and elastomers, as well as their origination from renewable resources and their complete biodegradability [1]. PHA can be divided into Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02118-9) contains supplementary material, which is available to authorized users. * Kanokphorn Sangkharak [email protected] 1
Department of Biology, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand
2
Research and Development Office, Prince of Songkhla University, Songkhla 90112, Thailand
3
Department of Agro and Bio Industry, Thaksin University, Phatthalung 93210, Thailand
4
Department of Chemistry, Faculty of Science, Thaksin University, Phatthalung 93210, Thailand
two groups, (i) short-chain-length PHA (scl-PHA) and (ii) medium-chain-length PHA (mcl-PHA), with 3–5 and 6–14 carbon atoms in their monomeric units, respectively [2]. The mcl-PHA and its copolymers are more suitable for a wide range of applications than scl-PHA due to their structural diversity, which allows for the tailoring of their phy
Data Loading...
