Bacillus tepidiphilus sp. nov., isolated from tepid spring
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ORIGINAL PAPER
Bacillus tepidiphilus sp. nov., isolated from tepid spring Manik Prabhu Narsing Rao1 · Zhou‑Yan Dong1 · Yu Kan2 · Min Xiao1 · Ying Qian Kang2 · Wen‑Jun Li1 Received: 23 February 2020 / Revised: 8 June 2020 / Accepted: 15 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel Bacillus strain, designated SYSU G01002T, was isolated from a sediment sample collected from tepid spring in Tengchong, Yunnan province, south-west PR China. The 16S rRNA gene sequence analysis showed that the strain SYSU G01002T shared the highest sequence identity with the type strain of Bacillus alkalitolerans (97.7%). Strain SYSU G01002T grew at pH 6.0–8.0 (optimum, pH 7.0), at 28–55 °C (optimum, 45 °C) and in the presence of 0–2.5% (w/v) NaCl (optimum in the absence of NaCl). It contained meso-2,6-diaminopimelic acid as the cell-wall diamino acid and MK-7 as isoprenoid quinone. The major cellular fatty acids were iso-C15:0, iso-C17:0 and C16:0. The polar were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified phospholipid. The genomic DNA G + C content was 38.0 mol %. The digital DNA–DNA hybridization and average nucleotide identity values between SYSU G01002T and closely related members of the genus Bacillus were below the cut-off level recommended for interspecies identity. Based on the above results, strain SYSU G01002T represents a novel species of the genus Bacillus, for which the name Bacillus tepidiphilus sp. nov. is proposed. The type strain, SYSU G01002T (= KCTC 43131T = CGMCC 1.17491T). Keywords Hot spring · Bacillus · Polyphasic taxonomy
Introduction
Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain SYSU G01002T is MN595122. The GenBank/EMBL/DDBJ accession numbers for genome sequence of SYSU G01002T and Bacillus alkalitolerans KCTC 33631T are WIAQ00000000 and JAAGVZ000000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01958-0) contains supplementary material, which is available to authorized users. * Wen‑Jun Li [email protected] 1
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People’s Republic of China
Key Laboratory of Medical Microbiology and Parasitology and Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Talent Base of Microbiology and Human Health of Guizhou Province, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 550025, People’s Republic of China
2
Hot springs once perceived to be sterile but the discovery of Thermus aquaticus (Brock 1997) and the valuable enzyme (Taq DNA polymerase) (Chien et al. 1976) suggest this environment not only holds hidden microorganisms but also valuable products. Tengchong (Yunnan Province of China) is the hotspot for the hot springs in China whic
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