Bacillus aerolatus sp. nov., a novel member of the genus Bacillus, isolated from bioaerosols in a school playground
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ORIGINAL PAPER
Bacillus aerolatus sp. nov., a novel member of the genus Bacillus, isolated from bioaerosols in a school playground Pei Chen1 · DeDong Wang2 · QiaoQiao Ren1 · JiGuo Wu1 · YunXia Jiang1 · ZhenDong Wu1 · YiMin Pan1 · Yi Zhong2 · Ying Guan1 · Kuncai Chen2 · GuoXia Zhang1 Received: 1 March 2020 / Revised: 30 May 2020 / Accepted: 15 June 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-positive, endospore-forming, rod-shaped bacterium with a single flagellum, and a motile strain, designated CX253, was isolated from bioaerosols. The isolate is facultatively anaerobic, is able to grow at 25–45 ℃ (optimum 37 ℃) and pH 6.5–10.0 (optimum 7.5), and can tolerate up to 5.0% NaCl (w/v) under aerobic conditions. The diagnostic diamino acid in the cell wall of strain CX253T is meso-diaminopimelic acid, while major isoprenoid quinone is menaquinone 6 (MK-6) along with a smaller amount of MK-7 (20%). The polar lipid profile is composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids and glycolipids. The major cellular fatty acid is iso-C15:0 and anteisoC15:0. Phylogenetic analysis based on 16S rRNA gene and genome sequence grouped strain CX253T into the genus Bacillus. The strain was most closely related to Bacillus thermotolerans CCTCC AB 2012108 T by comparison of 16S rRNA gene sequence (97.2% similarity) and to Bacillus wudalianchiensis CCTCC AB 2015266 T by comparison of gyrB gene sequence (80.1% similarity). The draft genome of strain C X253T comprised 3,929,195 bp with a G + C content of 43.3 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain CX253T and phylogenetically related Bacillus species were lower than 95% and 70%, respectively. Thus, the polyphasic evidence generated through phenotypic, chemotaxonomic and genomic methods confirmed that strain C X253T (= GDMCC 1.1608 T = KACC 21318 T) was a novel species of the genus Bacillus, for which the name Bacillus aerolatus sp. nov. is proposed. Keywords Bacillus aerolatus · Bioaerosols · 16S rRNA gene sequence · Genomic taxonomy
Introduction Communicated by Erko Stackebrandt. Pei Chen and DeDong Wang contributed equally to this work. GenBank accession numbers: The GenBank accession numbers for 16S rRNA gene and genome sequence of strain CX253T are MN442345 and WEIO00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01955-3) contains supplementary material, which is available to authorized users. * GuoXia Zhang [email protected] 1
Department of Environmental Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China
Guangzhou Center for Disease Control and Prevention, Guangzhou 510440, China
2
The genus Bacillus, a rod-shaped, endospore-forming, aerobic or facultatively anaerobic and Gram-positive bacteria, was first named by Cohn (1872). With improve
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