Bioconversion of 5-Hydroxymethylfurfural (HMF) to 2,5-Furandicarboxylic Acid (FDCA) by a Native Obligate Aerobic Bacteri
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Bioconversion of 5-Hydroxymethylfurfural (HMF) to 2,5-Furandicarboxylic Acid (FDCA) by a Native Obligate Aerobic Bacterium, Acinetobacter calcoaceticus NL14 Yequan Sheng 1,2,3 & Xin Tan 4 & Xin Zhou 1,2,3 & Yong Xu 1,2,3 Received: 23 January 2020 / Accepted: 23 April 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
2,5-Furandicarboxylic acid (FDCA), one of the top biomass-based platform chemical, is highly promising for resins and polymers, and it can be prepared from the bio-oxidation of hydroxymethyl furfural (HMF), which can be obtained mainly from lignocellulosic glucose that has a high production potential from not edible biomass. A native strain, Acinetobacter calcoaceticus NL14, that could convert HMF into FDCA is used for combining degradation and fermentation by consolidated bioprocessing (CBP). In this study, it was observed that the initial HMF concentration and pH neutralizer played important roles in the bioconversion of HMF, 5 g/L of HMF could be converted by 100% within 48 h with 0.5 g/L sodium carbonate (Na2CO3) with the production of 0.31 g/L FDCA. Extra glucose and hydrogen peroxide (H2O2) addition could further promote the production of FDCA to 0.54 g/L with 100% HMF conversion and a higher conversion rate. This report could provide a potential native bacterium for furan chemicals bioconversion and bioelimination, especially for FDCA bioproduction. Keywords 5-Hydroxymethylfurfural . Acinetobacter calcoaceticus NL14 . Bioconversion . 2,5Furandicarboxylic acid
* Yong Xu [email protected] Yequan Sheng [email protected] Xin Tan [email protected] Xin Zhou [email protected] Extended author information available on the last page of the article
Applied Biochemistry and Biotechnology
Introduction Biomass pretreatment generates a wide range of toxic compounds from lignin and carbohydrate degradation [1]. These toxic degradation compounds in lignocellulosic hydrolysates negatively affect the bioproduction performance of biofuels and biochemicals. 5Hydroxymethylfurfural (HMF) is one of the most widely accepted hexose degradation products from the lignocellulosic biomass pretreatment process. It is toxic to ethanol fermentation strains by attacking the cell membrane and interfering with intracellular metabolism [2]. With the furan ring, hydroxymethyl, and formyl functional groups, HMF is considered to be among the furan platform compounds and can be converted into various less toxic and more valuable chemicals such as 2,5-furandicarboxylic acid (FDCA) [3, 4], which has been listed in the top 10 value-added bio-based chemicals [5]. FDCA is considered as a “green” substitute replacing terephthalic acid (TPA) in the production of polyethylene terephthalate (PET) [6, 7]. Furthermore, FDCA has also been mentioned as one of the most promising biomass-based building blocks for the synthesis of polyesters, polyamides, and other valuable furan chemicals [3]. Nowadays, the market price of FDCA is on average $2300/kg; thus, the researches on synthesis of FDCA have increa
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