Biomimetic Cerium Oxide Loaded Gelatin PCL Nanosystems for Wound Dressing on Cutaneous Care Management of Multidrug-Resi
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ORIGINAL PAPER
Biomimetic Cerium Oxide Loaded Gelatin PCL Nanosystems for Wound Dressing on Cutaneous Care Management of Multidrug-Resistant Bacterial Wound Healing Yi Fei1 • Qiong Huang2 • Zheqing Hu3 • Xunv Yang3 • Baifen Yang3 • Shuang Liu4 Received: 28 March 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Skin wound healing involves a coordinated cellular response to achieve complete reepithelialization. Elevated levels of reactive oxygen species (ROS) in the wound environment often pose a hindrance in wound healing resulting in impaired wound healing process. Herein, cerium oxide nanoparticles (CeNPs) matrices like nanofibers have also been explored for enhancing wound healing. In this work, we have established the fabrication of CeNPs functionalized gelatin polycaprolactone (PCL) nanofibers (PCLNF) and CeNPs laden with PCL nanofiber (PCLNPNF) matrices by the electrospun methods. The bioactive composition PCLNPNF maintained the fiber morphological behaviour for up to 15 days. Further, the PCLNPNF matrices enhanced the cell proliferation against the NIH-3T3 cell lines established by Alamar blue assay. Furthermore, the PCLNPNF matrices scavenged ROS property examined with DCFH-DA intensity of the fluorescence microscopy. The antibacterial property of the PCLNPNF shows the remarkable activity against the gram-positive (S. aureus), and gram-negative (P. aeruginosa). Additionally, the in vivo bacterial removals and invented wound injuries examined development were definite through the MRSA-infects mice model. Taking to the advantages of this work, the newly fabricated PCLNPNF is a promising antibiotic alternative to battle the various bacterial infections in future. Keywords Cerium oxide nanoparticles Cell viability Multidrug-resistant bacteria Wound healing
Introduction
Yi Fei and Qiong Huang contributed equally to this work & Zheqing Hu [email protected] & Shuang Liu [email protected] 1
Wound Stoma Clinic, Affiliated Cixi Hospital, Wenzhou Medical University, No.999, Second South Ring East Road, Cixi 315300, China
2
Operation Room, Hangzhou First People’s Hospital Affiliated to Zhejiang University, Hangzhou 310006, China
3
Department of Nursing Care, Affiliated Cixi Hospital, Wenzhou Medical University, Ningbo 315300, China
4
Department of Interventional Vascular Surgery, The Fourth Affiliated Hospital of Harbin Medical University, No. 37, Yiyuan Street, Harbin 150001, China
Wound dressings are used to protect the wounds from infections, prevent dehydration, promote epithelial restoration and minimize the scarring. There are a wide variety of production techniques and materials available for the preparation of wound dressings [1–4]. Microbes are the major reason for infection and the prevalence of the same is high in and around us. The major infection-causing bacteria were Staphylococcus aureus (S. aureus) [5–8]. Previously entered into the humans, these microbial organisms establish quickly appears the colonies for
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