C-terminally encoded peptides (CEPs) are potential mediators of abiotic stress response in plants
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RESEARCH ARTICLE
C-terminally encoded peptides (CEPs) are potential mediators of abiotic stress response in plants Sakshi Aggarwal1 • Ashish Kumar1 • Muskan Jain1 • Jebi Sudan2 Kapil Singh1 • Sumita Kumari2 • Ananda Mustafiz1
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Received: 5 March 2020 / Revised: 18 August 2020 / Accepted: 4 September 2020 Ó Prof. H.S. Srivastava Foundation for Science and Society 2020
Abstract Intracellular signaling is a critical determinant of the normal growth and development of plants. Signaling peptides, also known as peptide hormones, along with classical phytohormones, are the significant players of plant intracellular signaling. C-terminally encoded peptide (CEP), a 15-amino acid post-translationally peptide identified in Arabidopsis, plays a pivotal role in lateral root formation, nodulation, and act as long-distance root to shoot signaling molecule in N-starvation conditions. Expression of CEP gene members in Arabidopsis is perturbed by nitrogen starvation; however, not much is known regarding their role in other abiotic stress conditions. To gain a comprehensive insight into CEP biology, we identified CEP genes across diverse plant genera (Glycine max, Sorghum bicolor, Brassica rapa, Zea mays, and Oryza sativa) using bioinformatics tools. In silico promoter analysis revealed that CEP gene promoters show an abundance of abiotic stress-responsive elements suggesting a possible role of CEPs in abiotic stress signaling. Spatial and temporal expression patterns of CEP via RNA seq and microarray revealed that various CEP genes are transcriptionally regulated in response to abiotic stresses. Validation of rice CEP genes expression by qRT-PCR showed that
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12298-020-00881-4) contains supplementary material, which is available to authorized users. & Ananda Mustafiz [email protected] 1
Plant Molecular Biology Laboratory, Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi 110021, India
2
School of Biotechnology, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu 180009, India
OsCEP1, OsCEP8, OsCEP9, and OsCEP10 were highly upregulated in response to different abiotic stress conditions. Our findings suggest these CEP genes might be important mediators of the abiotic stress response and warrant further overexpression/knockout studies to delineate their precise role in abiotic stress response. Keywords Abiotic stress C-terminally encoded peptide (CEP) qRT-PCR Signaling peptides Abbreviations CEP C-terminally encoded peptide AA Amino acid CAREs Cis-acting regulatory elements qRT-PCR Quantitative real-time polymerase chain reaction ABA Abscisic acid ACC 1-Aminocyclopropane 1-carboxylic acid JA Jasmonic acid SA Salicylic acid
Background Intracellular communication and coordination among different tissues and cells is an essential determinant for the growth and development of multicellular organisms. In animals, cell to cell communication primarily involves signaling peptides like i
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