Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Bi
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Characterization of an L-Arabinose Isomerase from Bacillus velezensis and Its Application for L-Ribulose and L-Ribose Biosynthesis Zongren Guo 1,2,3 & Liangkun Long 1,2,3 & Shaojun Ding 1,2,3 Received: 10 February 2020 / Accepted: 23 June 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn2+. Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribose from L-arabinose using a co-expression system harboring L-Al and L-RI. Keywords Bacillus velezensis, L-Arabinose isomerase, L-Ribulose, L-Ribose co-expression
* Shaojun Ding [email protected]; [email protected] Zongren Guo [email protected] Liangkun Long [email protected]
1
The Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China
2
Jiangsu Key Lab for the Chemistry & Utilization of Agricultural and Forest Biomass, Nanjing Forestry University, Nanjing 210037, China
3
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Applied Biochemistry and Biotechnology
Introduction Rare sugars are defined as the monosaccharides and their derivatives which hardly exist in nature [1, 2]. Rare sugars have numerous physiological properties, and show broad applications in the food, cosmetics, and pharmaceutical industries [3, 4]. LRibulose is a rare pentose that can be used as a precursor for the production of highvalue sugars such as L-ribose. L-Ribose has been widely used as initial substrate in the synthesis of L-nucleoside derivatives, which have commonly played significant roles in the production of antiviral agents and anticancer drugs [5–7]. Because Lribose displays excellent commerce and application value, industrial production for Lribose has attracted broad attention in recent years. It has been reported that L-ribose can be obtained by several chemical synthesis approaches [8, 9], but the drawbacks of chemical synthesis are tedious and sophisticated. Moreover, some unexpected by-products often appeared in the processes of chemical reaction [10]. In contrast, the enzymatic methods for synthesis L-ribose have many advantages such as great efficiency, stereoselectivity, and easy to operate [11]. These advantages make enzymatic processes become desirable to replace traditional chemical methods in the industry. L-Arabinose is a natural monosaccharide that can be easily
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