CircRNA0001859, a new diagnostic and prognostic biomarkers for COPD and AECOPD
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RESEARCH ARTICLE
Open Access
CircRNA0001859, a new diagnostic and prognostic biomarkers for COPD and AECOPD Shuifang Chen* , Yinan Yao, Shan Lu, Junjun Chen, Guangdie Yang, Lingfang Tu and Lina Chen
Abstract Background: Dysregulation of circRNAs has been reported to be functionally associated with chronic obstructive pulmonary disease (COPD). The present investigation elucidated the potential role of CircRNA0001859 in regulating chronic obstructive pulmonary disease acute (COPD) and Acute Exacerbation of COPD (AECOPD). Methods: Mice model of COPD was established to screen and verify the dysregulated expression of CircRNA0001859. Fluorescence in situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR) were carried out to detect the expression of CircRNA0001859. 38 stable COPD patients, 24 AECOPD patients, 57 COPD with lung cancer patients and 28 healthy person with age and sex matched to total patients were used for the present investigation. Results: circRNA0001859 was downregulated in the lung tissue of mice after the three kinds of treatments (Cigarette smoke (CS)/NK alone or CS + NNK) for inducing COPD. FISH assay verified the downregulation of circRNA0001859 both in the mice lung and human bronchial epithelial cell of COPD model. Furthermore,, the level of circRNA0001859 was also downregulated in the peripheral blood of COPD and lung cancer patients. CircRNA0001859 might act as a diagnostic and prognostic biomarker for the treatment of in COPD and AECOPD with Are under the receiver operating characteristic curve (ROC curve) (AUC) of 0.7433 and 0.8717, respectively. Conclusion: We explored a novel circRNA0001859, which might act as a potential therapeutic biomarker for the treatment of COPD and AECOPD. Keywords: Circular RNA0001859 (CircRNA0001859), CS, NNK, Chronic obstructive pulmonary disease (COPD), Acute exacerbation of COPD (AECOPD), Lung cancer
Background Chronic obstructive pulmonary disease (COPD), a heterogeneous disorder is estimated to be the third leading cause of chronic morbidity and mortality worldwide [1]. COPD is associated with severe coronavirus disease 2019 (COVID-19) [2]. Global Burden of Diseases, Injuries and Risk factors study (GBD) 2017, reported that COPD lead to an estimated death of 3.2 million people * Correspondence: [email protected] The First Affiliated Hospital, College of Medicine, Zhejiang University, NO.79, Qingchun Road, Hangzhou 310003, China
worldwide [3]. China Pulmonary Health (CPH) study on 50,991 individuals (21,446 men and 29, 545 women) with reliable post-bronchodilator results revealed prevalence of spirometry-defined COPD to be 8.6% which is accounting for 99.9 million people with COPD in China and seen higher COPD rates in men (11.9%) than in women (5.4%) and in people aged 40 years or older (13.7%) than in those aged between 20 and 39 years; the major risk of COPD in Chinese population includes cigarette smoking, ambient air pollution, underweight,
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0
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