Collagen and elastin scaffold by electrospinning for skin tissue engineering applications
- PDF / 662,539 Bytes
- 9 Pages / 584.957 x 782.986 pts Page_size
- 94 Downloads / 205 Views
tituto Politécnico Nacional, Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, C.P. 11500 Ciudad de México, México Address all correspondence to this author. e-mail: [email protected]
a)
Received: 21 March 2019; accepted: 26 June 2019
In recent years, tissue engineering has helped to reduce hospital stays and deaths caused by skin wounds. Scaffolds are one of the main factors that influence the success of any tissue graft. Collagen is one of the main components of the extracellular matrix, and there has been much interest in new sources for application as a biomaterial. In this work, a tissue engineering scaffold was developed using the electrospinning technique. The chicken skin was used as an alternative source to obtain collagen. The combination of this collagen with elastin was successfully electrospun, and a distribution of diameters was obtained, less than 100 nm. In vitro tests showed the adhesion and proliferation of the cells, as well as an absence of cytotoxicity from non–crosslinked scaffolds and scaffolds that were cross-linked with carbonyldiimidazole. The structure and composition of the developed scaffolding provide a favorable environment for cell growth and generating a skin substitute.
Introduction The skin is the largest organ of the human body and protects it from the external environment. Its main functions are the regulation of temperature, sensation, water conservation, and immunological protection [1]. Skin wounds with a total area of more than 4 cm2 must be treated with a graft. When the healing process is not adequate, a wound can become chronic. Chronic wounds may not heal and lead to amputations or mortality [2]. Recent biotechnological progress suggests that tissue engineering approaches have the potential to neutralize tissue and organ deficiencies [3]. One of the main factors that influence the success of a graft is the type of tissue engineering scaffolding that is used. Some of the main criteria for the design of a tissue engineering scaffold for a specific type of tissue are the adhesion, infiltration, proliferation, and cell differentiation, as well as the development of new tissue in three dimensions [4]. Different techniques have been reported for the development of a functional scaffold for skin tissue engineering. Membranes synthesized by electrospinning have been classified as an optimal scaffolding option with useful biological and mechanical properties. Electrospun nanofibers have exceptional properties such as a similar structure to the natural extracellular matrix (ECM),
ª Materials Research Society 2019
permeability, and the regulation scar formation [5, 6]. Collagen is the most abundant protein in the ECM. This biomaterial is one of the most effective in the engineering of various tissues. The triple helix structure of collagen has a high content of the amino acid glycine and is very important for cell attachment and proliferation [7]. Extracellular fibrous networks formed by the combination of collagen, elastin, and keratin allow tissues and organs to with
Data Loading...
