Comparative analysis of intraspecific variability in yellow banded wasp, Vespa cincta F. (Vespidae: Hymenoptera) using m
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ORIGINAL RESEARCH ARTICLE
Comparative analysis of intraspecific variability in yellow banded wasp, Vespa cincta F. (Vespidae: Hymenoptera) using molecular and morphometric techniques Denisha Rajkhowa 1
&
Mukul Kumar Deka 2 & Basanta Bora 3 & Anjumoni Devee 2 & Ataur Rahman 2
Received: 9 June 2020 / Accepted: 23 October 2020 # African Association of Insect Scientists 2020
Abstract The yellow banded wasp, Vespa cincta F. is one of the most destructive pests of honeybee colonies in India. Despite high species richness, there is diminutive information on the intraspecific variations occurring in the wasp from different geographical regions. The present study was undertaken to ascertain if any intraspecific variability existed in populations of V. cincta from four geographical regions of Assam, India. The adult specimens from each region were studied using 24 morphological characters, as well as with genetic markers subjected to PCR. No significant variation was recorded for 15 morphological characters; however, nine characters viz., body length, thorax length and width, mandible length and width, fore wing width, fore leg width, hind leg width, and sting width were significantly larger in specimens collected from Barak Valley and Hill Zone (BVHZ) as compared to specimens from Upper Brahmaputra Valley Zone (UBVZ), Lower Brahmaputra Valley Zone (LBVZ) and North Bank Plain Zone (NBPZ). Variability in morphometrics further revealed that species from UPVZ, LBVZ, and NBPZ occupied similar positions, with a clear distinction from the species of BVHZ. Molecular analyses further supplemented this information with the SSR based dendrogram readily separating V. cincta into two main clusters, A and B. Cluster A included the species from LBVZ, NBPZ, and UBVZ while cluster B comprised of species from BVHZ only. Within NBPZ, specimens from Nagaon and Morigaon regions differed to some extent from specimens of other regions. Specimens of Jorhat region of UBVZ were found to more closely related to specimens of Sonitpur region of NBPZ while specimens of Golaghat region of UBVZ formed a separate sub-cluster with specimens of Karbi Anglong region of BVHZ. The present findings suggest that intraspecific diversity in V. cincta could be a result of variation in evolutionary lineage as well as environmental aspects of their bio-geographical area of origin. Keywords Vespa cincta . Assam . Geographic . Molecular . Dendrogram . Wasp
Introduction Integrative insect taxonomy uses molecular and morphometric information to accurately establish hypotheses related to evolutionarAcanski et al. 2016y patterns (Bakkes et al. 2020),
* Denisha Rajkhowa [email protected] 1
Department of Entomology, College of Agriculture, Central Agricultural University, Pasighat, Arunachal Pradesh 791102, India
2
Department of Entomology, College of Agriculture, Assam Agricultural University, Jorhat, Assam 785013, India
3
Department of Biotechnology, College of Agriculture, Assam Agricultural University, Jorhat, Assam 785013, India
species boundaries (
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