Comparative transcriptome analysis of gynoecious and monoecious inflorescences reveals regulators involved in male flowe
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ORIGINAL ARTICLE
Comparative transcriptome analysis of gynoecious and monoecious inflorescences reveals regulators involved in male flower development in the woody perennial plant Jatropha curcas Mei‑Li Zhao1,3 · Mao‑Sheng Chen1,2 · Jun Ni1 · Chuan‑Jia Xu1,3 · Qing Yang1 · Zeng‑Fu Xu1,2 Received: 8 August 2019 / Accepted: 17 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message ABCE model genes along with genes related to GA biosynthesis and auxin signalling may play significant roles in male flower development in Jatropha curcas. Abstract Flowering plants exhibit extreme reproductive diversity. Jatropha curcas, a woody plant that is promising for biofuel production, is monoecious. Here, two gynoecious Jatropha mutants (bearing only female flowers) were used to identify key genes involved in male flower development. Using comparative transcriptome analysis, we identified 17 differentially expressed genes (DEGs) involved in floral organ development between monoecious plants and the two gynoecious mutants. Among these DEGs, five floral organ identity genes, Jatropha AGAMOUS, PISTILLATA, SEPALLATA 2-1 (JcSEP2-1), JcSEP2-2, and JcSEP3, were downregulated in ch mutant inflorescences; two gibberellin (GA) biosynthesis genes, Jatropha GA REQUIRING 1 and GIBBERELLIN 3-OXIDASE 1, were downregulated in both the ch and g mutants; and two genes involved in the auxin signalling pathway, Jatropha NGATHA1 and STYLISH1, were downregulated in the ch mutant. Furthermore, four hub genes involved in male flower development, namely Jatropha SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 1, CRYPTOCHROME 2, SUPPRESSOR OF OVEREXPRESSION OF CO 1 and JAGGED, were identified using weighted gene correlation network analysis. These results suggest that floral organ identity genes and genes involved in GA biosynthesis and auxin signalling may participate in male flower development in Jatropha. This study will contribute to understanding sex differentiation in woody perennial plants. Keywords Comparative transcriptome analysis · Flower development · Gynoecy · Monoecy · Physic nut · Sex differentiation and determination
Introduction
Communicated by Zhenbiao Yang. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00497-020-00396-8) contains supplementary material, which is available to authorized users. * Mao‑Sheng Chen [email protected] * Zeng‑Fu Xu [email protected] 1
CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Innovation Academy for Seed Design, Chinese Academy of Sciences, Menglun, Mengla 666303, Yunnan, China
Land plants have extremely diverse sexual systems (Barrett 2002; Henry et al. 2018). In flowering plants, sex differentiation or determination is a developmental decision that occurs during the life cycle of each plant and leads to the differentiation of male (stamens) and female (carpels) 2
Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences,
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