Considerations for large-scale implementation of dormant budwood cryopreservation
- PDF / 2,047,960 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 50 Downloads / 123 Views
REVIEW
Considerations for large‑scale implementation of dormant budwood cryopreservation Justin D. Tanner1 · Katheryn Y. Chen1 · Remi M. Bonnart2 · Ioannis S. Minas1 · Gayle M. Volk2 Received: 1 May 2020 / Accepted: 1 July 2020 © Springer Nature B.V. 2020
Abstract Cryopreservation of clonal plant germplasm is a reliable way to preserve important agronomic traits and protect against loss of crop genetic diversity of many horticultural species. Dormant bud cryopreservation techniques present an efficient alternative to the labor-intensive shoot tip cryopreservation process and may allow a single technician to preserve large quantities of germplasm in a season. This method of cryopreservation takes advantage of the natural dormancy in cold hardy crops, making it a viable technique for only deciduous trees and shrubs. Many factors must be considered when attempting to perform dormant bud research methods to applied-level germplasm preservation efforts. This process is necessarily a seasonal endeavor, which puts strain on labor and facilities particularly in winter. Integration of methods and procedures using different crop species or new equipment provides additional challenges that must be tested in advance. By identifying variables of dormant bud processing in cryopreservation literature, options emerge that allow for the modification of reported methods to work within the confines of institutional resources. Infrastructure, pre-processing, and recovery stages are discussed in terms of necessity and available alternatives to allow informed decision making in establishing an applied dormant budwood genebank. Key message This review aims to provide information about the DB cryopreservation method with a focus on its many variables and permutations reported in literature to allow genebanks and germplasm managers to make informed decisions regarding the designation of critical resources. Keywords Dormant budwood · Clonal genebanking · Applied cryopreservation · Tree fruit germplasm · Liquid nitrogen storage Abbreviations DB Dormant bud(s) H Hours HQC 8-Hydroxyquinoniline citrate
LN Liquid nitrogen LNV Liquid nitrogen vapor MC Moisture content TTC Triphenyltetrazolium chloride
Communicated by Ranjith Pathirana.
Introduction
* Justin D. Tanner [email protected] * Gayle M. Volk [email protected] 1
Department of Horticulture & Landscape Architecture, Colorado State University, Fort Collins, CO 80523, USA
National Laboratory for Genetic Resources Preservation, Agricultural Research Service, United States Department of Agriculture, 1111 S. Mason St., Fort Collins, CO 80521, USA
2
Germplasm conservation is essential to preserving the genetic diversity of cultivated and wild species. Clonal plant collections can successfully maintain specific genetic combinations of highly heterozygous crops, like fruit trees, that rely on asexual propagation. While clonal collections may be maintained in field plots, greenhouses, screenhouses, and in vitro culture labs, long-term preservation in cryogeni
Data Loading...
