Coping with stress: role of Arabidopsis phytoglobins in defence against Sclerotinia sclerotiorum
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ORIGINAL ARTICLE
Coping with stress: role of Arabidopsis phytoglobins in defence against Sclerotinia sclerotiorum Nitika Mukhi1,3
•
Suman Kundu2 • Jagreet Kaur1
Received: 2 April 2020 / Accepted: 22 September 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract Phytoglobins (Pgbs) are multifaceted stress-responsive proteins implicated in regulating various physiological and stressresponsive pathways in plants. Previous work has demonstrated NO dioxygenase and peroxidase-like activity of Arabidopsis phytoglobin 3 (AHb3) and its potential role in defense against Sclerotinia sclerotiorum. The work reported here highlights the significance of the other two classes of Arabidopsis phytoglobins (AHb1 and AHb2) in response to S. sclerotiorum. Constitutive expression of AHb1 (OEAHb1) and AHb2 (OEAHb2) conferred marginal tolerance towards S. sclerotiorum whereas respective knockdown (RNAi) lines displayed enhanced susceptibility, with AHb1 RNAi (RNAi-1) lines being more susceptible in comparison to AHb2 RNAi (RNAi-2) lines. Interestingly, transgenic lines with a simultaneous reduction in the transcripts of AHb1 and AHb2 (RNAi-F) displayed greater disease spread in comparison to individual knockdown lines indicative of their additive effect. The enhanced susceptibility upon pathogen challenge correlated with the elevated NO and H2O2 levels in these lines. Furthermore, detailed structural analysis hints towards an alternate mechanism of NO dioxyegnation by AHbs. Taken together, the current investigation illustrates the NO dioxygenase and peroxidase-like activity of AHbs and highlights their role in defense against stem rot pathogen S. sclerotiorum. Keywords Arabidopsis phytoglobins Sclerotinia sclerotiorum Nitric oxide dioxygenase peroxidase Abbreviations Pgbs Phytoglobins AHb Arabidopsis phytoglobin NO Nitric oxide ROS Reactive oxygen species OE Overexpression H2O2 Hydrogen peroxide.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13562-020-00615-3) contains supplementary material, which is available to authorized users. & Jagreet Kaur [email protected] 1
Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India
2
Department of Biochemistry, University of Delhi South Campus, New Delhi 110021, India
3
Present Address: Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, UK
Introduction Three classes of phytoglobin genes (Pgbs) can be distinguished in plant genomes. Each class displays unique kinetic and structural fingerprints (Trevaskis et al. 1997; Watts et al. 2001). Depending upon the plant species, Pgbs are expressed across diverse plant organs throughout all developmental stages and display huge diversity in their expression profiles ( Hunt et al. 2001; Garrocho-Villegas et al. 2007; Hebelstrup et al.2007; Bacana et al. 2020). The presence of phytoglobin genes in metabolically active and stressed tissue implicates their role in plant development as well as in
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