Designing of a stable and selective glucose biosensor by glucose oxidase immobilization on glassy carbon electrode sensi
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RESEARCH ARTICLE
Designing of a stable and selective glucose biosensor by glucose oxidase immobilization on glassy carbon electrode sensitive to H2O2 via nanofiber interface Zülfikar Temoçin1 Received: 15 June 2020 / Accepted: 27 October 2020 © Springer Nature B.V. 2020
Abstract The integration of the enzymes on the solid electrode surfaces is an indispensable step for the construction of the bioelectrochemical electrode. In the current work, the blend nanofibers consisting of poly (vinyl alcohol) and poly(ethyleneimine) were deposited by the electrospinning method on an H2O2-sensitive modified glassy carbon electrode. Glucose oxidase was immobilized on the glutaraldehyde-activated blend nanofibers. Bioelectrochemical electrode displayed a good linear response to the glucose concentration ranges with two separate calibration curves, from 2 to 8 mmol L−1 and from 10 to 30 mmol L −1. Besides, it showed a high anti-interference performance against ascorbic and uric acids as well as long-term storage stability over 63 days. Moreover, analysis results in a diluted human serum sample showed that the prepared bioelectrochemical electrode has the ability to measure glucose in real samples. Graphic abstract
Keywords Glassy carbon · Glucose oxidase · Nanofiber · Glucose biosensor · Poly(vinyl alcohol) Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10800-020-01502-4) contains supplementary material, which is available to authorized users. * Zülfikar Temoçin [email protected] 1
Department of Chemistry, Faculty of Arts and Sciences, Kırıkkale University, Yahşihan, 71450 Kırıkkale, Turkey
1 Introduction In recent years, much research has been conducted over bioelectrochemical electrodes (BEC), which are employed for the recognition of various target analytes [1–3]. These constructed electrodes often employ one or more enzymes to exploit the functions tendered by natural structures of
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enzymes. Especially, reduction–oxidation enzymes have been considerably used in biosensor development due to their ability to catalyze reactions based on electron transfer [4–6]. There are two major challenges to the construction of the BECs: (i) assembly of the enzyme onto an electrode surface [7, 8] and (ii) communication between the immobilized enzyme and the electrode [9–11]. Simple immobilization techniques are used to assemble an enzyme on the electrode surface and to provide the necessary microenvironments for the protection of the sensitive structure of the enzymes [7, 12, 13]. Enzymes have been assembled on the electrode by different immobilization techniques such as physical attachment [14, 15], entrapment [16], layer-by-layer assembly [17, 18], and covalent attachment [19]. Among these techniques, the advantage of covalent immobilization is minimized leakage of the immobilized enzyme from the electrode surface, which leads to an increase in the stability of the BEC [20, 21]. Nanofibers are used as support materials because they have a high sur
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