Differences in pre-imaginal development of the honey bee Apis mellifera between in vitro and in-hive contexts
- PDF / 1,469,612 Bytes
- 15 Pages / 467.716 x 680.315 pts Page_size
- 14 Downloads / 218 Views
Original article
Differences in pre-imaginal development of the honey bee Apis mellifera between in vitro and in-hive contexts Diego E. VÁZQUEZ1,2 , Walter M. FARINA1,2 1
Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, Laboratorio de Insectos Sociales, Universidad de Buenos Aires, Buenos Aires, Argentina 2 Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina Received 14 June 2019 – Revised 11 February 2020 – Accepted 30 March 2020
Abstract – In vitro rearing of honey bees is becoming a widely employed procedure. Nevertheless, few studies have assessed its impact on brood development considering nutritional and social differences between both in vitro and in-hive rearing contexts. We compared developmental parameters between these two contexts using an intraframe grafting procedure. We confirmed a grafting effect on development. However, pre-imaginal survival did not depend on the rearing procedure. The correlation in mortality between groups of larvae (exposed or not to grafting) was independent of the rearing context suggesting transmission of inheritable and infectious factors. In addition, the comparison between contexts showed that in vitro larvae had delayed molting and achieved emergence 1.57 days later. Furthermore, in vitro imagoes emerged with lower weight and shorter wing length than in-hive bees. Our results strengthen the idea that there is an in vitro phenotype as a consequence of phenotypic plasticity. Apis mellifera / larval rearing / brood grafting / phenotypic plasticity / inter-colony variability
1. INTRODUCTION The increasing disturbance of the agricultural ecosystems negatively affects the health of pollinators as they have to constantly adapt to various environmental challenges. Insect pollinators, including native and managed bees, have declined in abundance and diversity on local and regional scales in North-West Europe and the American continent (IPBES 2016; Kluser et al. 2010; Potts et al. 2010; vanEngelsdorp and Meixner 2010). The decline in honey bee (Apis mellifera ) Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13592-020-00767-0) contains supplementary material, which is available to authorized users.
Corresponding author: W. FARINA, [email protected] Manuscript editor: Yves Le Conte
population is well-documented and is thought to be a consequence of multiple concomitant factors, such as exposure to monocultures, agrochemicals, pathogens, parasites, extreme climate conditions, and bad beekeeping practices (IPBES 2016; Kluser et al. 2010). Open-field risk assessments of hives focused on the bias of brood development (Wu et al. 2011) cannot establish causality to a single factor. Additionally, they are not able to assess dose-response relationships due to the complexity of in-hive exposure. As a result, the in vitro rearing procedure (details in Sections 2.2 and 2.3) has been developed as a way to asses
Data Loading...
