Dynamics of volatilomes emitted during cross-talking of plant-growth-promoting bacteria and the phytopathogen, Fusarium
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(2020) 36:152
ORIGINAL PAPER
Dynamics of volatilomes emitted during cross‑talking of plant‑growth‑promoting bacteria and the phytopathogen, Fusarium solani A. Gutiérrez‑Santa Ana1 · H. A. Carrillo‑Cerda1 · J. Rodriguez‑Campos2 · J. B. Velázquez‑Fernández3 · O. A. Patrón‑Soberano4 · S. M. Contreras‑Ramos1 Received: 19 May 2020 / Accepted: 5 September 2020 © Springer Nature B.V. 2020
Abstract The dynamics of volatilomes emitted during the interaction between plant-growth-promoting bacteria (PGPB) and the phytopathogen Fusarium solani were evaluated for 5 days. The first screening was done to evaluate the antagonist activity of volatile compounds emitted by PGPB against F. solani. Volatilomes from 11 PGPB were determined individually and together with F. solani by using solid-phase microextraction coupled to gas-chromatography–mass spectrometry. Isolates of PGPB belonged to the Bacillus genus and inhibited from 18 to 24% the fungal mycelium growth. The isolates also induced morphological alterations of fungal hyphae, like small globular vesicles and the formation of chlamydospores, suggesting a stress mechanism response by the fungus. Volatilome profile showed 49 different compounds that appeared in the bacterial–fungal interaction, such as ketones, sesquiterpenes, monoterpenoids, alkanes, alkenes, carboxylic acids, and fatty acids. Some ketones and alcohols were detected in high abundance only in the interaction PGPB-fungus at 3 and 5 days. Bacillus circulans A19, Bacillus amyloliquefaciens A21, and Bacillus wiedmannii S18 shared a group of emitted alcohols and ketones when they were exposed to F. solani. F. solani produced its own volatilome profile, with the presence of sesquiterpenes, such as α-cubebene and caryophyllene, which increased significantly in co-incubation with the tested bacteria, suggesting chemical communication between them.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11274-020-02928-w) contains supplementary material, which is available to authorized users. * J. Rodriguez‑Campos [email protected] * S. M. Contreras‑Ramos [email protected] 1
Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Av. Normalistas No. 800, Col. Colinas de La Normal, 44270 Guadalajara, Jalisco, Mexico
2
Unidad de Servicios Analíticos Y Metrológicos (USAM), CIATEJ, Av. Normalistas No. 800, Col. Colinas de La Normal, 44270 Guadalajara, Jalisco, Mexico
3
Catedra-Conacyt assigned to Unidad de Tecnología Ambiental at CIATEJ, Guadalaja, Jalisco, Mexico
4
División de Biología Molecular, Instituto Potosino de Investigación Científica Y Tecnológica A.C. (IPICYT), Camino a la Presa San José 2055, Lomas 4ª. Sección, 78216 San Luis Potosí, San Luis Potosí, Mexico
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World Journal of Microbiology and Biotechnology
(2020) 36:152
Graphic abstract
Keywords Bacillus spp. · Bacterial volatile compound · Biological control · Volatile pr
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