Effect of light quality and tissue origin on phenolic compound accumulation and antioxidant activity in Camellia japonic
- PDF / 3,278,618 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 67 Downloads / 211 Views
BIOACTIVE/MEDICINAL COMPOUNDS
Effect of light quality and tissue origin on phenolic compound accumulation and antioxidant activity in Camellia japonica calli Eun Bi Jang 1,2 & Thanh-Tam Ho 3,4 & So-Young Park 1 Received: 4 March 2020 / Accepted: 1 September 2020 / Editor: Yong Eui Choi # The Society for In Vitro Biology 2020
Abstract In Camellia japonica callus culture, the effects of light quality on the accumulation of phenolic compounds were examined. To this end, the calli were cultured under three monochrome [white (W), red (R), and blue (B)] and two mixed [red/blue (RB) and red/green/blue (RGB)] light sources. After 4 weeks of culture, fresh and dry callus weights were determined, and phenolic content was analyzed using high-performance liquid chromatography. We also examined the effects of RGB light on calli derived from four origins (leaf: CL; root: CR; petal: CP; and ovary: CO). Total phenolic and flavonoid content and DPPH radical scavenging activity were highest in calli exposed to RB and RGB. Catechin was detected only in calli exposed to mixed light (RB and RGB). Interestingly, red pigmentation development in CL and CR calli after 2 weeks of culture and organogenic calli in CR and CP samples was observed. Furthermore, expression of the phenylalanine ammonia lyase and chalcone synthase genes was found to be higher in the CR line than in other lines, and total phenolic and flavonoid content was also higher in the CR and CP lines. The findings of the present study revealed that the accumulation of phenolics and flavonoids in callus tissue varies depending on light quality, which stimulates cell division, and may thus affect the proliferation and organogenesis of C. japonica callus. We also established that the effects of light on red pigment expression and the accumulation of bioactive compounds are dependent on the tissue origin of callus material. Keywords Antioxidant activity . Callus culture . Mixed light sources . Phenolics . Flavonoids
Introduction Camellia japonica (Theaceae) is an evergreen tree distributed in East Asia, where it is mainly grown for ornamental purposes (Li et al. 2016). The twigs, leave, and fruits of camellia contain catechin, epicatechin, cyanidin, and other bioactive compounds, and recent studies have demonstrated the antioxidant activity and efficacy of these compounds such as * So-Young Park [email protected] 1
Department of Horticultural Science, Division of Animal, Horticulture and Food Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea
2
Biodiversity Research Institute, Jeju Technopark, Shinyedong-ro 338, Seogwipo-si, Jeju 63608, Republic of Korea
3
Institute for Global Health Innovations, Duy Tan University, Da Nang 550000, Vietnam
4
Faculty of Pharmacy, Duy Tan University, Da Nang 550000, Vietnam
antiatherogenic effect (Lee et al. 2016; Chen et al. 2018; Páscoa et al. 2019). However, under field cultivation, the content of various C. japonica metabolites can differ markedly depending on the season, and the cultivation area is generally
Data Loading...
