Effective microbiological decontamination of dental healing abutments colonised with Rothia aeria by a diode laser as a
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ORIGINAL ARTICLE
Effective microbiological decontamination of dental healing abutments colonised with Rothia aeria by a diode laser as a helpful step towards successful implantoprosthetic therapy Anna Wawrzyk 1 & Mansur Rahnama 2 & Dorota Rybitwa 3
&
Sławomir Wilczyński 4 & Monika Machoy 5 & Michał Łobacz 2
Received: 27 March 2020 / Accepted: 22 September 2020 # Springer-Verlag London Ltd., part of Springer Nature 2020
Abstract The aim of the study was to find variant of diode laser (λ = 810 nm) irradiation, which ensures elimination of unwanted microorganisms, including Rothia aeria, from dental healing abutments, and consequently accelerates process of wound healing in implantologically treated patients. The scope of the study included identification of the most contaminated areas on healing abutments, identification of microorganisms inhabiting various environments of oral cavities, assessment of effectiveness of various laser decontamination parameters against detected microorganisms (preliminary studies) and assessment of wound healing in patients after applying abutments with low roughness and optimal variant of laser irradiation (clinical studies). Imaging of surfaces of the healing abutments was performed using vertical scanning interferometry, scanning electron microscopy and optical microscopy. Microorganisms inhabiting the healing abutments, teeth and saliva from tested patients were identified using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Three programmes of near-infrared diode laser at average powers of 1.00–3.84 W with two variants of exposure time were used for optimisation of laser parameters. Observation of wound healing was performed for 100 patients during 20 days after installation of abutments. On surfaces of the used healing abutments, a large number of microorganisms, with a predominance of R. aeria, were found. Irradiation with periimplantitis surgical programme for 30 s resulted in 99–100% reduction in the number of R. aeria and other microorganisms, depending on type of abutment (in vivo). The use of diode laser in the selected variant accelerates wound healing and provides complete elimination of pathogenic R. aeria and other microorganisms inhabiting surfaces of the healing abutments without marks. Keywords Healing abutments . Diode laser . Decontamination . Rothia aeria . Microorganisms
Introduction The oral cavity is a natural habitat for many bacteria and fungi. Most of the microorganisms inhabiting oral cavity do not
* Dorota Rybitwa [email protected] 1
Sanitary-Epidemiological Station, Prądnicka 76, 31-202 Kraków, Poland
2
The Chair and Department of Oral Surgery, Medical University of Lublin, Chodźki 6, 20-093 Lublin, Poland
3
Medical Laboratory ‘Labmed’, 11-Listopada 3e/2, 32-600 Oświęcim, Poland
4
Department of Basic Biomedical Sciences, Medical University of Silesia, Kasztanowa 3, 41-200 Sosnowiec, Poland
5
Division of Orthodontics, Pomeranian Medical University in Szczecin, Powstańców Wielkopolskich 72, 70-111 Szczecin
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