Effects of Percutaneous Collagen Induction Therapy Associated with Hyaluronic Acid on Inflammatory Response, Oxidative S
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ORIGINAL ARTICLE
Effects of Percutaneous Collagen Induction Therapy Associated with Hyaluronic Acid on Inflammatory Response, Oxidative Stress, and Collagen Production Maria Eduarda Anastácio Borges Corrêa,1 Daniela Pacheco dos Santos Haupenthal,1 Carolini Mendes,1 Rubya Pereira Zaccaron,1 Laura de Roch Casagrande,1 Ligia Milanez Venturini,1 Germano Duarte Porto,1 João Vitor Silvano Bittencourt,1 Jennyffer Ione de Souza Silva,2 Samara de Sousa Mariano,2 Thiago Antônio Moretti de Andrade ,2 and Paulo Cesar Lock Silveira 1,3,4
Abstract— Percutaneous collagen induction (PCI) is an alternative treatment for skin dys-
functions, it aims to stimulate collagen production by encouraging normal wound healing that occurs after any trauma by inducing microlesions; also it may be potentiated with the association with drugs such as hyaluronic acid (HA). Our objective was to evaluate the effects of PCI associated with hyaluronic acid (0.9%) on inflammatory process, oxidative stress, and collagen production in rat epidermis. For the study, 36 adult Wistar rats were randomly divided into 6 groups (n = 6): Control; PCI 0.5; PCI 1.0; HA; PCI 0.5 + HA; and PCI 1.0 + HA. The animals were anesthetized, trichotomized, and the application of therapies was performed once; After 7 days, the animals were euthanized for removal of the skin region. Levels of pro-inflammatory (IL1, IL6, TNFα), anti-inflammatory (IL4 and IL10) cytokines and growth factors (FGF, TGFβ) were evaluated, besides oxidative stress parameters and histological analysis. In combination groups, there is a decrease in TNFα compared with the control and PCI groups in contrast to a significant increase in anti-inflammatory cytokines and growth factors. Oxidant and oxidative damage levels showed a significant
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10753-020-01291-0) contains supplementary material, which is available to authorized users. 1
Laboratory of Experimental Phisiopatology, Program of postgraduate in Sciences of Health, Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina state 88806-000, Brazil 2 Graduate Program of Biomedical Sciences, University Center of Herminio Ometto Foundation - FHO, Araras, SP, Brazil 3 Av. Universitária, 1105 Universitário – Block S, Room 017, Criciúma, SC CEP: 88806-000, Brazil 4 To whom correspondence should be addressed at Av. Universitária, 1105 Universitário – Block S, Room 017, Criciúma, SC CEP: 88806-000, Brazil. E-mail: [email protected]
0360-3997/20/0000-0001/0 # 2020 Springer Science+Business Media, LLC, part of Springer Nature
decrease in PCI + HA groups in relation to PCI groups while antioxidant defense increased in PCI + HA groups compared with the control group. The number of fibroblasts was increased in the PCI 1.0 group in relation to the control, HA, and PCI 0.5. The number of blood vessels and collagen area was increased in groups PCI and PCI + HA compared with the HA group. We conclude that the combination of PCI with HA is able to accelerate t
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