Evolution and expression analysis of CDPK genes under drought stress in two varieties of potato
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ORIGINAL RESEARCH PAPER
Evolution and expression analysis of CDPK genes under drought stress in two varieties of potato Zhenzhen Bi . Yihao Wang . Pengcheng Li . Chao Sun . Tianyuan Qin . Jiangping Bai
Received: 8 May 2020 / Accepted: 22 October 2020 Ó Springer Nature B.V. 2020
Abstract Objectives Calcium-dependent protein kinases (CDPKs) function directly in plant development and stress responses. We used whole genome sequences and mRNA expression data to analyze the phylogenetic relationships, gene structure, collinearity, and differential expression of CDPKs in two differentially drought-tolerant potato varieties. Results In total, we identified 25 CDPK proteins belonging to four subfamilies. There was a significant collinear relationship among 13 CDPK genes belonging to four segmentally duplicated pairs. Subcellular prediction implied that all StCDPKs were localized at the plasma membrane. Analysis of promoter regions revealed that StCDPKs were photosensitive and responsive to biotic stress, abiotic stress, and hormone stimuli. RNA-seq analysis showed differential Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10529-020-03037-2) contains supplementary material, which is available to authorized users. Z. Bi Y. Wang P. Li C. Sun T. Qin J. Bai (&) Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China e-mail: [email protected] Z. Bi e-mail: [email protected] Z. Bi Y. Wang P. Li C. Sun T. Qin J. Bai College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China
expression of StCDPKs among various potato tissues, and qPCR analysis revealed that 20 StCDPKs exhibited differential expression patterns under drought stress between drought-tolerant (QS9) and drought sensitive (Atl) potato varieties. Among these, the most strongly drought-induced genes were respectively StCDPK3 and StCDPK23, highlighting these as attractive candidate genes for further functional analyses of drought-stress responses in potato. Conclusions Our results demonstrating the tissue specific and drought stress-responsive StCDPK genes of potato both provide a reference for further research about the functions of CDPK family proteins and should support ongoing efforts for the further genetic improvement of potato. Keywords CDPK Phylogenetic analysis Stress response Drought
Introduction The Ca2?-dependent protein kinases (CDPKs/CPKs) function directly in various physiological processes in plants, including growth and development and abiotic and biotic stress responses. Several CDPKs are essential in abiotic stress tolerance and function by modulating abscisic acid (ABA) signaling and reducing the accumulation of reactive oxygen species
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Biotechnol Lett
(ROS), and positively or negatively regulating stress tolerance. Phosphoproteomics analysis revealed that SnRK2s and CDPKs/CPKs share substrates in ABA signaling (Umezawa et al. 2013). The downstream pathways of ABA signaling CPK6, CPK21, and CP
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