Expression of CysLTR1 and 2 in Maturating Lymphocytes of Hyperplasic Tonsils Compared to Peripheral Cells in Children
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ORIGINAL ARTICLE
Expression of CysLTR1 and 2 in Maturating Lymphocytes of Hyperplasic Tonsils Compared to Peripheral Cells in Children Bruno Peres Paulucci,1,5 Juliana Pereira,2 Patricia Picciarelli,3 Debora Levy,4 and Renata Cantisani di Francesco1
Abstract—Cysteinyl-leukotriene receptors 1 and 2 (CysLTR1 and 2) are related to allergic inflammatory responses. Recent studies demonstrated their role in lymphocyte division and maturation in the bone marrow. Few data are available about CysLTRs function in lymphocyte maturation in tonsils. The objectives of this study are to compare CysLTRs expression in peripheral blood lymphocytes with expression in maturating lymphocytes of hyperplasic tonsil and to check the influence of respiratory allergies in this process. Leukocytes of peripheral blood (PL) and hyperplasic tonsils of children were immunostained for CysLTR1, CysLTR2, CD3 (T cells), and CD19 (B cells) and read in flow cytometer. Lymphocyte of tonsils were divided in differentiating small cells (SC) and mitotic large cells (LC); percentage of B and T cells expressing CysLTRs was determined, and comparison was done using ANOVA and Tukey’s tests. Data were analyzed as a whole and categorizing patients according the presence of allergies. Sixty children were enrolled in this study. There was a large expression of CysLTR1 and 2 in CD3+ LC, and such expression decreased progressively in SC and PL. In B cells, the highest expression of CysLTR1 and 2 was found in PL while SC showed the lowest and LC showed the intermediate expression. This pattern kept unchanged in groups of allergic and non-allergic individuals. CysLTRs seem to be involved in lymphocyte maturation that occurs in tonsils, without influence of allergies. New studies aiming the clinic treatment of tonsil hyperplasia must be targeted to the development of drugs capable of blocking both CysLTR1 and 2. KEY WORDS: lymphocytes; leukotriene; allergy; tonsil; maturation.
INTRODUCTION Tonsils are secondary immunologic organs that start their development in the first year of life and reach the highest size between the fourth and eighth years [1]. Its abnormal enlargement that occurs in 3–5 % of children generally is symptomatic and is the key factor in the pathophysiology of sleep respiratory disturbances (SRD), serous otitis, and disturbance in facial development [2]. These patients typically show follicular hyperplasia pattern [3], high mitotic activity of centroblasts, centrocytes overpopulation, expansion of germinal centers, and enlargement of subcapsular areas [3, 4]. The starting stimulus for this process remains unknown, but recent
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Department of Otolaryngology of Clinics Hospital, University of Sao Paulo—Brazil, Av. Dr. Eneas de Carvalho Aguiar, 255 - 6° andar - sala 6167, 05403-000 Sao Paulo, SP, Brazil 2 Department of Hematology of Clinics Hospital, University of Sao Paulo—Brazil, Sao Paulo, SP, Brazil 3 Department of Pathology of Clinics Hospital, University of Sao Paulo—Brazil, Sao Paulo, SP, Brazil 4 Laboratory of Research in Hematology of Clin
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