Genome-wide characterization, evolution and expression profiling of UDP-glycosyltransferase family in pomelo ( Citrus gr
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RESEARCH ARTICLE
Open Access
Genome-wide characterization, evolution and expression profiling of UDPglycosyltransferase family in pomelo (Citrus grandis) fruit Boping Wu1, Xiaohong Liu2, Kai Xu1*
and Bo Zhang2*
Abstract Background: Pomelo is one of the three major species of citrus. The fruit accumulates a variety of abundant secondary metabolites that affect the flavor. UDP-glycosyltransferases (UGTs) are involved in the glycosylation of secondary metabolites. Results: In the present study, we performed a genome-wide analysis of pomelo UGT family, a total of 145 UGTs was identified based on the conserved plant secondary product glycosyltransferase (PSPG) motif. These UGT genes were clustered into 16 major groups through phylogenetic analysis of these genes with other plant UGTs (A-P). Pomelo UGTs were distributed unevenly among the chromosomes. At least 10 intron insertion events were observed in these UGT genome sequences, and I-5 was identified to be the highest conserved one. The expression profile analysis of pomelo UGT genes in different fruit tissues during development and ripening was carried out by RNA-seq. Conclusions: We identified 145 UGTs in pomelo fruit through transcriptome data and citrus genome database. Our research provides available information on UGTs studies in pomelo, and provides an important research foundation for screening and identification of functional UGT genes. Keywords: UDP-glycosyltransferase, Expression analysis, Pomelo, Evolution, Glycosylation
Background Plants produce a variety of secondary metabolites that are involved in important mechanisms at different developmental stages. As an important modification reaction, glycosylation is usually the last step in the biosynthesis of secondary metabolites in plants. In coordination with methylation, hydroxylation, and acylation, glycosylation * Correspondence: [email protected]; [email protected] 1 The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China 2 Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology / Laboratory of Fruit Quality Biology, Zhejiang University, Hangzhou 310058, China
contributes to the diversity and complexity of secondary metabolites in plants [1]. Glycosylation is the main mechanism for stabilizing and enhancing the solubility of metabolites in plants, thereby regulating plant signaling pathways and intracellular homeostasis [2, 3]. Glycosyltransferase catalyzes glycosylation [4]. In 2020, a total of 110 glycosyltransferase (GT) super families were included in the CAZy database (http://www.cazy. org), of which the GT1 family had the largest number of gene members and is mainly in plants. The GT1 family is commonly referred to as UDP-glycosyltransferase (UGT), because primarily it catalyzes the transfer of UDP-sugars to specific receptors, including plant
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons
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