Genome-wide identification, characterization and expression analysis of non-RD receptor like kinase gene family under Co
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ORIGINAL PAPER
Genome‑wide identification, characterization and expression analysis of non‑RD receptor like kinase gene family under Colletotrichum truncatum stress conditions in hot pepper R. Srideepthi1 · M. S. R. Krishna1 · P. Suneetha2 · R. Sai Krishna3 · S. Karthikeyan1 Received: 9 October 2019 / Accepted: 8 September 2020 © Springer Nature Switzerland AG 2020
Abstract Receptor like kinases (RLKs) are preserved upstream signaling molecules which regulate several biological processes from plant development to various stress adaptation programs. Non arginine aspartate (non-RD) a prominent class of RLKs plays a significant role in disease resistance and apoptosis in plants. In present investigation, a comprehensive in silico analysis for non-RD Kinase gene family as well as identification of gene structures, sequence similarity, chromosomal localization, gene duplication analysis, promoter analysis, transcript expression profiles and phylogenic studies were done. In this study, twenty-six genes were observed on nine out of twelve chromosomes. All these genes were clustered into five subfamilies under large monophyletic group termed as Interleukin-1 Receptor-Associated Kinase (IRAK) family. Some of the important physiochemical properties of twenty-six proteins are determined and ranged in the following order: (a) Amino acids size ranged from (620 to 1781) (b) Molecular weight ranged as of (70.11 to 197.11 KDa) and (c) Theoretical PI ranged from (5.69 to 8.63) respectively. Structural diversity in genomic structure among non-RD kinase gene family was identified and presence of pathogen induced cis regulatory elements including STRE, MYC, MYB, and W box were found. Expression profiles revealed the potential ability of three genes CaRLK1 from LRRXII and CaRLK15,16 from stress antifung subfamily were pointedly upregulated beyond the severe stress time period (9 DAI) in anthracnose resistant genotype PBC-80 in response to Colletotrichum truncatum infection. Subsequently, in silico studies from the available genome sequencing data helped us to identify candidate genes tangled in inducing disease resistance. Keywords Pattern recognition receptors (PRRs) · Auto phosphorylation · Downstream signaling · Colletotrichum truncatum · Defense responses
Introduction One of the major challenges in the twenty-first century, to worldwide food security and agricultural sustainability, is to develop economically important high yielding varieties that are stable with broad-spectrum of resistance. Hot pepper is Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10709-020-00104-4) contains supplementary material, which is available to authorized users. * M. S. R. Krishna [email protected] 1
Koneru Lakshmaiah Education Foundation, Guntur, India
2
Professor Jaya Shanker Telangana State Agricultural University, Hyderabad, India
3
ICAR- National Rice Research Institute, Cuttack, India
a commercially important vegetable crop grown worldwide for its indispensable nutrition
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