Genotypic characterisation and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients of diff
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RESEARCH ARTICLE
Open Access
Genotypic characterisation and antimicrobial resistance of Pseudomonas aeruginosa strains isolated from patients of different hospitals and medical centres in Poland Marcin Brzozowski1, Żaneta Krukowska1, Katarzyna Galant2, Joanna Jursa-Kulesza1 and Danuta Kosik-Bogacka3* Abstract Background: Pseudomonas aeruginosa is a Gram-negative bacteria responsible for infections in immunocompromised patients and is one of the most common causes of nosocomial infections particularly in intensive care and burn units. We aimed to investigate the population structure of P. aeruginosa strains isolated from patients at different hospital wards. Methods: We analysed the possible presence of P. aeruginosa epidemic or endemic strains in hospitals of the selected region. A genotyping analysis was performed for P. aeruginosa isolates (n = 202) collected from patients of eleven hospitals in north-western Poland. Collections of P. aeruginosa were genotyped using pulsed-field gel electrophoresis (PFGE). Phenotypic screening for antibiotic susceptibility was performed for the common antimicrobial agents. Results: Pseudomonas aeruginosa isolates were distributed among 116 different pulsotype groups. We identified 30 groups of clonally related strains, each containing from 2 to 17 isolates and typed the obtained 13 unique patterns, designated as A, D, E, J, K, M, N, Ó, P, T, X, AC, AD, and AH. The two largest clusters, D and E, contained 17 and 13 isolates, respectively. Strains of these groups were continuously isolated from patients at intensive care units and burn units, indicating transmission of these strains. Conclusions: In this study, we demonstrate the clonal relatedness of P. aeruginosa strains and their constant exchange in hospitals over a period of 15 months. The obtained results indicate a predominantly non-clonal structure of P. aeruginosa. Keywords: Pseudomonas aeruginosa, Genotyping, Antimicrobial resistance, PFGE, Population structure
Background Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen that causes nosocomial infections for patients with pre-existing lung disease, including cystic fibrosis or chronic obstructive pulmonary disease, or patients on mechanical ventilation in the intensive care (ICUs). Eradication of P. aeruginosa in hospitals is especially problematic due to its intrinsic resistance to many * Correspondence: [email protected] 3 Independent of Pharmaceutical Botany, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, 70-111 Szczecin, Poland Full list of author information is available at the end of the article
antibiotic classes and its capacity to acquire resistance to all effective antibiotics [1, 2]. Pseudomonas aeruginosa colonization of patients may originate from different exogenous sources such as sanitary installations (sinks, hot tubs, showers, etc.), contaminated diagnostic devices, mechanical ventilation, and cleaning equipment [3]. Infections of healthy individuals are rather rare; however, healthy colonised patients can serv
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