Hair follicle stem cells differentiation into bone cells on collagen scaffold
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Hair follicle stem cells differentiation into bone cells on collagen scaffold Saeideh Aran . Saber Zahri . Asadollah Asadi . Fatemeh Khaksar . Arash Abdolmaleki
Received: 14 October 2019 / Accepted: 24 January 2020 Ó Springer Nature B.V. 2020
Abstract The hair follicle is a dynamic structure which contains different niches for stem cells, therefore; it has been considered as valuable and rich sources of stem cells, due to easy access, multipotency and non-oncogenic properties. In the present study, the differentiation capacities of hair follicle stem cells into bone cells on the natural collagen scaffolds were investigated. The stem cells were extracted from the hair follicle bulge area of male Wistar rats’ whisker and cultured until 3rd passage, then osteogenic differentiations were induced by culturing the cells in the specific osteogenic medium. After 3 weeks, the differentiation parameters, including morphological changes, levels of calcification and expression of the bone specific genes were detected. The hydrogel preparation and scaffold fabrication was carried out using the extracted collagen and was studied by scanning electron microscope. Comparison of the S. Aran S. Zahri (&) A. Asadi F. Khaksar Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran e-mail: [email protected] A. Abdolmaleki Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Iran A. Abdolmaleki Bio Science and Biotechnology Research Center (BBRC), Sabalan University of Advanced Technologies (SUAT), Namin, Iran
stem cells’ growth and changes on the scaffold and non-scaffold conditions showed that, in the both situation, the cells revealed differentiation signs of osteocytes, including large and cubic morphology with a star-shaped nucleus. Staining by Alizarin-red and Von-Kossa methods showed the presence of red and black calcium mass on the scaffold. Expression of the osteopontin and alkaline phosphatase genes confirmed the differentiation. Considerable porosity in the surface of the scaffold was recorded by scanning electron microscopy, which made it convenient for cells’ attachment and growth. The data showed that the bulge stem cells possess significant capacity for osteoblastic differentiation and collagen scaffolds were found to be an appropriate matrix for growth and differentiation of the cell. Keywords Hair follicle stem cells Collagen hydrogel Osteoblasts Bone
Introduction The hair follicle is one of the skin’s appendixes, which originates from the Epithelial-mesenchymal interactions during embryonic development (Peters et al. 2017; Sasaki 2019). Stem cells are undifferentiated cells that divide during their lives and can repair damaged tissues. These cells have two basic characteristics, including self-renewal and the ability to
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differentiate into diverse types of cells (Kikuchi et al. 2011). Hair follicle stem cells are located in an
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