Hepatoprotective Effects of Selenium-Enriched Probiotics Supplementation on Heat-Stressed Wistar Rat Through Anti-Inflam
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Hepatoprotective Effects of Selenium-Enriched Probiotics Supplementation on Heat-Stressed Wistar Rat Through Anti-Inflammatory and Antioxidant Effects Rahmani Mohammad Malyar 1,2,3 & Emal Naseri 4 & Hu Li 1,2 & Ilyas Ali 1 & Rawan Ahmad Farid 3 & Dandan Liu 1,2 & Khushdil Maroof 3 & Maazullah Nasim 4 & Sayed Attaul Haq Banuree 3 & Kehe Huang 1,2 & Kevin J. Waldron 5 & Xingxiang Chen 1,2 Received: 11 August 2020 / Accepted: 3 November 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The purpose of this study was to elucidate the effects of selenium-enriched probiotics on the liver of heat-stressed Wistar rats. Ten-week-old male rats were assigned to four groups: control (Con); high temperature (HT); high temperature plus probiotics (HT + P: 1011 CFU/mL Lactobacillus acidophilus and 109 CFU/mL Saccharomyces cerevisiae); or high temperature plus selenium-enriched probiotics (HT + SeP: 0.3 mg/kg Se, 1011 CFU/mL L. acidophilus and 109 CFU/ mL S. cerevisiae). The HT, HT + P, and HT + SeP groups were maintained at higher ambient temperature (40–42 °C), while the control group was kept at room temperature (25 °C). After 42 days of thermal exposure, blood and liver tissues were collected and analyzed for morphological and molecular markers of liver physiology. The body weight of rats in the HT group decreased but liver weight and live index were increased. Histological examination showed dilation of liver sinusoids and congestion of interstitial veins in HT group. Moreover, the histomorphology of the liver in HT + P and HT + SeP groups was restored, and the serum AST, ALT, ALP, LDH, and hepatic MDA level decreased significantly, but the serum total protein level and the liver SOD, T-AOC, and GSH-PX activities were increased significantly relative to the HT group. In addition, the mRNA level of Gpx1, SOD1, Nrf2, and Bcl-2 was significantly increased, while the expression level of Bax, IL-6, TNF-α, COX-2, NF-κB, α-SMA, TGFβ1, Collagen I, HSP70, and HSP90 was significantly decreased in liver tissues after SeP supplementation. We concluded that SeP can protect Wistar rats from oxidative stress, inflammation, apoptosis, and liver fibrosis induced by heat stress. Keywords Liver . Selenium-enriched probiotics . High ambient temperature . Inflammation . Oxidative stress, . Apoptosis . Wistar rats
Introduction * Xingxiang Chen [email protected] 1
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
2
Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowl, Nanjing Agricultural University, Nanjing 210095, China
3
Faculty of Veterinary Science, Nangarhar University, Jalalabad, Nangarhar Province, Afghanistan
4
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Science and Technology/College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
5
Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, UK
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