Identification of ACE inhibitory peptides from red alga Mazzaella japonica
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ORIGINAL PAPER
Identification of ACE inhibitory peptides from red alga Mazzaella japonica Yuya Kumagai1 · Yumi Kitade2 · Manami Kobayashi2 · Kei Watanabe2 · Hiroki Kurita2 · Hirohumi Takeda3 · Hajime Yasui4 · Hideki Kishimura1 Received: 28 April 2020 / Revised: 3 July 2020 / Accepted: 4 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Angiotensin I converting enzyme (ACE) inhibition is one of the key factors for cardiovascular disease, and inhibition of ACE activity is related to the prevention of high blood pressure. Until now, some ACE inhibitory peptides have been found in the protein hydrolysates of red algae, and most of them were derived from phycobiliproteins and rubisco. In this study, we modified the preparation method to evaluate the potential of ACE inhibitory activity from protein hydrolysate of red alga Mazzaella japonica except for phycobiliproteins and rubisco. As a result, we identified 11 peptides (YRD, VSEGLD, TIMPHPR, GGPAT, SSNDYPI, SRIYNVKSNG, VDAHY, CPYDWV, YGDPDHY, NLGN and DFGVPGHEP) in the hydrolysate by the reversed phase-HPLC and MALDI-TOF/MS/MS. Among them, YRD would be derived from phycoerythrin α-subunit. However, the others would be from other proteins encoded in chloroplast or nuclear genomes. This study revealed that not only phycobiliproteins and rubisco but also the other proteins in red algae have the potential for the source of ACE inhibitory peptide. Keywords ACE inhibitory activity · Antihypertension · Red alga · Mazzaella japonica · Protein hydrolysate
Introduction High blood pressure (a systolic blood pressure equal to or higher than 140 mmHg and a diastolic blood pressure equal to or higher than 90 mmHg) is a worldwide problem, because patient is estimated to be more than one billion people, in other word, one in three adults aged 25 and older [1]. High blood pressure has been thought one of the risk
* Hideki Kishimura i‑[email protected] 1
Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041‑8611, Japan
2
Chair of Marine Chemical Resource Development, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041‑8611, Japan
3
Hokkaido Research Organization, Fisheries Research Department, Abashiri Fisheries Research Institute, 7‑8‑5 Minato‑cho Monbetsu, Hokkaido 094‑0011, Japan
4
Laboratory of Humans and the Ocean, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041‑8611, Japan
factors for cardiovascular diseases, and angiotensin I converting enzyme (ACE; EC 3.4.15.1) plays an important role in the regulation of blood pressure [2]. There are some antihypertensive drugs including captopril, enalapril, lisinopril and alacepril, but these chemically synthesized inhibitors usually associated with unacceptable side effects such as dry cough, angioedema, taste disturbance and skin rash [3, 4]. Therefore, ACE inhibitory peptides derived from nature sources are desirable for prevention of hypertension. P
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