Characterization of Labrenzia polysiphoniae sp. nov. isolated from red alga Polysiphonia sp.
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ORIGINAL PAPER
Characterization of Labrenzia polysiphoniae sp. nov. isolated from red alga Polysiphonia sp. Lyudmila A. Romanenko1 · Valeriya V. Kurilenko1 · Konstantin V. Guzev1 · Vassilii I. Svetashev2 Received: 24 August 2018 / Revised: 21 January 2019 / Accepted: 20 February 2019 © Springer-Verlag GmbH Germany, part of Springer Nature 2019
Abstract A group of five Gram-negative aerobic halophilic bacteria was isolated from the red alga Polysiphonia sp. specimen collected from the Sea of Japan seashore and subjected to a taxonomic study. On the basis of 16S rRNA gene sequence analysis, the novel isolates were affiliated to the genus Labrenzia sharing the highest gene sequence similarities of 98.1–98.4% with the type strain of Labrenzia suaedae KACC 13772T. The DNA–DNA hybridization values of 83–91% obtained between five novel strains, and 26 and 36% between two of the five novel strains and the closest neighbor Labrenzia suaedae KACC 1 3772T confirmed their assignment to the same separate species. Novel isolates were characterized by Q-10 as the major ubiquinone, by the predominance of C18:1ω7c followed by 11-methyl C18:1ω7c and C 14:0 3-ОН in their fatty acid profiles. Polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, an unknown aminophospholipid, and an unknown phospholipid. Some of novel strains were found to inhibit growth of Gram-negative and Gram-positive test microorganisms. On the basis of phylogenetic analysis, DNA–DNA hybridization and phenotypic traits, a novel species with the name Labrenzia polysiphoniae sp. nov. (type strain KMM 9 699T = rh46T = KACC 19711T), is proposed. Keywords Labrenzia polysiphoniae sp. nov. · Marine · Red alga · Polysiphonia sp.
Introduction The genus Labrenzia was proposed by (Biebl et al. 2007) for Labrenzia alexandrii as the type species of the genus and three species of the genus Stappia (Uchino et al. 1998) reclassified as Labrenzia aggregata, Labrenzia marina, and
Communicated by Erko Stackebrandt. The DDBJ/GenBank/EMBL accession number of the 16S rRNA gene sequence of strain KMM 9699T is LC381921. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-019-01640-0) contains supplementary material, which is available to authorized users.
Labrenzia alba, and emended by Bibi et al. (2014). Two species, Labrenzia suaedae (Bibi et al. 2014) and Labrenzia salina (Camacho et al. 2016), were subsequently described. At the time of writing the genus Labrenzia comprises six recognized species, which have been isolated from sea water, marine sediments, dinoflagellates, oysters, and halophytes (Kim et al. 2006; Biebl et al. 2007; Bibi et al. 2014; Camacho et al. 2016). In the present study, the taxonomic position of five novel strains isolated from the red alga Polysiphonia sp. specimen collected from the Sea of Japan seashore was defined. On the basis of the phenotypic characteristics, DNA–DNA relatedness and phylogenetic analysis the nove
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