Identification of novel microRNAs for cold deacclimation in barley
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ORIGINAL PAPER
Identification of novel microRNAs for cold deacclimation in barley Fei Chen1 · Jun He2 · Gulei Jin2 · Zhong‑Hua Chen3 · Fei Dai2 Received: 3 April 2020 / Accepted: 21 July 2020 © Springer Nature B.V. 2020
Abstract Cold acclimation is crucial for the overwintering process of plants. Cold deacclimation is also important for plant survival in winter, which results in loss of freezing tolerance and initiation of growth. MicroRNAs (miRNAs) play crucial roles in regulating various physiological activities including cold response in plants. However, there is no study on miRNAs and their target genes in response to cold deacclimation in a cold-tolerant crop – barley (Hordeum vulgare L.). Here, we performed high-throughput sequencing of miRNAs of leaves during the cold deacclimation process using two barley cultivars with contrasting cold tolerance (Nure, tolerant and Tremois, sensitive). We found a total of 36 known and 267 novel miRNAs, including 12 known and 112 novel ones that are differentially expressed during cold deacclimation. The number of detected differentially expressed miRNAs was larger in Nure than that in Tremois, and the expression profile of miRNAs was dramatically different between Nure and Tremois. Moreover, we identified 13 known and 97 novel miRNAs, which have putative target genes during cold deacclimation. The putative targets of the novel miRNAs included genes encoding C-repeat binding factor (CBF) transcription factors, phytohormones, antioxidant, osmopretectant and flower development. Our results suggest that barley miRNAs respond quickly to cold deacclimation, and the larger number of miRNAs differentially expressed in the cold tolerant cv. Nure indicating that miRNAs might play an important role in the process of deacclimation. It sets a solid foundation for future studies and breeding programs on low temperature tolerance in barley. Keywords Hordeum vulgare L. · High-throughput sequencing · microRNAs · Cold deacclimation · Cold acclimation · Cold tolerance
Introduction Cold tolerance of plants increases with a period of exposure to low non-freezing temperature, which is known as cold acclimation (Thomashow 1999). Cold acclimation is Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00646-9) contains supplementary material, which is available to authorized users. * Fei Chen [email protected] * Fei Dai [email protected] 1
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
2
Department of Agronomy, Zhejiang Key Lab of Crop Germplasm, Zhejiang University, Hangzhou 310058, China
3
School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW 2753, Australia
important for the development of cold tolerance in plants, which involves alterations of gene expression and many biochemical and physiological changes (Thomashow 1999; Zhu 2016). In contrast, cold treated plants lose their freezing tolerance after a short per
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