Impact of sildenafil-containing ointment on wound healing in healthy and experimental diabetic rats
- PDF / 1,132,401 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 10 Downloads / 191 Views
ORIGINAL ARTICLE
Impact of sildenafil‑containing ointment on wound healing in healthy and experimental diabetic rats Wael Abu Dayyih1 · Walid Abu Rayyan2 · Hisham Y. Al‑Matubsi2 Received: 30 April 2020 / Accepted: 17 June 2020 © Springer-Verlag Italia S.r.l., part of Springer Nature 2020
Abstract Aim The study evaluated the effect of different concentrations of sildenafil-containing ointment on wound healing in healthy and streptozotocin (STZ)-induced diabetic rats. Methods A total of 108 Sprague Dawley male rats aged 5 months were randomly divided into two groups: healthy and diabetes-inducing rats. Following induction of diabetes by intraperitoneal STZ injection (55 mg/kg), diabetic and healthy rats were subdivided into six groups (9 rats each). Linear incisions of the dorsal backs of rats were made. Then, rats were treated twice daily with either 2%, 2% + Fucidin, 5% sildenafil-containing ointments, oral sildenafil (13.0 mg/kg), moist exposed burn ointment or vehicle. During treatment, the percent reduction of the wound area and tensile strength were measured on days 3, 7 and 10. Furthermore, histopathology was performed on the wounded skin on similar days for the assessment of collagen synthesis and proliferation of new capillary vessels. Results In healthy and STZ-induced diabetic rats, the percent reduction in wound area on day 3 was significantly higher in sildenafil-containing ointment-treated groups than all other groups, whereas on day 7 only the 5% sildenafil-containing ointment-treated group showed better response in healthy rats. Although the sildenafil-containing ointment significantly showed better tensile strength than all other groups, the 5% sildenafil-treated group significantly enhanced the tensile better than the 2% and 2% + Fucidin dose. Furthermore, the histological evaluation revealed that sildenafil-containing ointment promoted collagen synthesis and proliferation of new capillary vessels. Conclusion Our results suggest that sildenafil-containing ointment can provide an advantage in wound healing by promoting wound contractions and resistance to wound breakage in healthy and diabetic conditions. Therefore, 5% sildenafil-containing ointment can be used as a support factor for wound healing in healthy and diabetic conditions; however, clinical trials are required to confirm the benefits of sildenafil in wound healing. Keywords Sildenafil ointment · Wound healing · Diabetic · Tensile strength
Introduction Managed By Massimo Porta. * Hisham Y. Al‑Matubsi [email protected] Wael Abu Dayyih [email protected] Walid Abu Rayyan [email protected] 1
Department of Pharmaceutical Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan
Department of Pharmacology and Medical Sciences, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan
2
Wound healing is a physiological process that follows tissue damage. The process continues until the wound area diminishes, and the tensile strength of th
Data Loading...
