In Vitro and In Vivo Anti-diabetic Activity of Fractions Obtained from the Unexplored Hedychium coronarium Rhizome
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RESEARCH ARTICLE
In Vitro and In Vivo Anti-diabetic Activity of Fractions Obtained from the Unexplored Hedychium coronarium Rhizome Suchitra Kumari Panigrahy1 • Awanish Kumar2 • Renu Bhatt1
Received: 12 August 2018 / Revised: 29 April 2019 / Accepted: 15 July 2019 The National Academy of Sciences, India 2019
Abstract Despite the availability of numerous medicines for diabetes treatment, compounds derived from plants are gaining popularity due to their lesser side effects. The present investigation was carried out to determine the antidiabetic activity of a terpenoid-rich fraction obtained from the rhizomes of Hedychium coronarium under in vitro and in vivo. The authors performed in vitro assays to determine glucose uptake in insulin-resistant cell lines. Further, the effect of terpenoid fraction was tested using albino Wistar rats. The streptozotocin-induced diabetic rats were treated with different concentrations of most active fraction, and results were compared with the standard drug insulin. At regular intervals, the blood glucose level and the bodyweight of the rats were monitored. The serum, biochemical and histological parameters related to the liver and kidney were also estimated. The fraction at 10 mg/kg.b.wt. has shown a significant reduction in blood glucose level and an increase in bodyweight. This specific concentration increases the activity of antioxidant enzymes, but reduces the activity of carbohydrate-metabolizing enzymes leading to normal blood glucose level. The normal cellular
Significance Statement The terpenoid fractions obtained from rhizomes of Hedychium coronarium have shown a significant antidiabetic activity both in vivo and in vitro.
architecture of liver and kidney tissue was also retained. The significant observations obtained from both in vitro and in vivo studies suggest the potential role of terpenerich fraction obtained from H. coronarium rhizomes in the treatment of diabetes. It also inferred that the fraction controls diabetes by regulating various parameters associated with diabetes-related complications. Keywords Anti-diabetic H. coronarium Streptozotocin (STZ) Terpenoid Carbohydrate-metabolizing enzyme Abbreviations STZ Streptozotocin ROS Reactive oxygen species DMEM Dulbecco’s modified eagle’s medium DMSO Dimethyl sulphoxide FBS Fetal bovine serum OGTT Oral glucose tolerance test SOD Superoxide dismutase CAT Catalase GR Glutathione reductase GST Glutathione S transferase TBARS Thiobarbituric acid reactive substances
& Awanish Kumar [email protected]; [email protected] & Renu Bhatt [email protected] 1
Department of Biotechnology, Guru Ghasidas Vishwavidyalaya (A Central University), Bilaspur, Chhattisgarh 495009, India
2
Department of Biotechnology, National Institute of Technology (NIT), Raipur, Chhattisgarh 492010, India
Introduction Diabetes mellitus (DM) is a chronic metabolic disorder caused due to inheritance or acquired deficiency in insulin secretion. This disorder affects about 5% of the global population and becomes a challenge for t
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