Antidiabetic and Antioxidant Activity of Green Synthesized Starch Nanoparticles: An In Vitro Study
- PDF / 2,469,775 Bytes
- 10 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 293 Views
(0123456789().,-volV)(0123456789(). ,- volV)
ORIGINAL PAPER
Antidiabetic and Antioxidant Activity of Green Synthesized Starch Nanoparticles: An In Vitro Study Vishnupriyan Varadharaj1 • Arulvel Ramaswamy2 • Rajeswari Sakthivel1 • Ramasamy Subbaiya2 Hamed Barabadi3 • Murugesan Chandrasekaran4 • Muthupandian Saravanan5
•
Received: 18 October 2019 Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract This study was aimed to assess the antidiabetic and antioxidant activity of starch nanoparticles (StNPs) synthesized by green synthesis through in vitro model. Several organic solvents were used for the extraction of active compounds from Gymnema sylvestre. The methanolic extract was used for the synthesis of StNPs from dried potato starch. The characterization studies such as GC–MS, FT-IR, SEM, EDS and XRD were performed to analyze the sample. The StNPs were found to be ellipsoidal in nature with the average particle size of 19.8 nm. The StNPs showed highest DPPH radical scavenging activity with 74.41 ± 0.54% at 100 lg/mL concentration. Besides, the IC50 values were found to be 66.69 lg/ mL and 61.99 lg/mL for the StNPs and standard (ascorbic acid), respectively. The StNPs showed the highest metal ion chelating activity of 66.71 ± 0.34% at a concentration of 100 lg/mL. The highest reducing power of StNPs was found to be 0.385 ± 0.002 at 100 lg/mL. Moreover, in vitro antidiabetic activity was studied using alpha-amylase inhibition assay and the highest inhibition activity of StNPs was 58.56 ± 0.44% at a concentration of 100 lg/mL. The present study provided preliminary evidence indicated that the StNPs synthesized using methanolic extract of G. sylvestre had potential activity against diabetes. Keywords Biosynthesis Starch nanoparticles Gymnema sylvestre Bioactive compounds Antidiabetic Antioxidant
Introduction Nanoscience and nanotechnology refer to the study and application of materials at nanoscale in different areas including biology, chemistry, physics, medicine, etc. [1, 2]. An estimated US$ 75.8 Billion has been anticipated for the
& Arulvel Ramaswamy [email protected] & Muthupandian Saravanan [email protected]; [email protected] 1
Department of Biotechnology, K.S.Rangasamy College of Technology, Namakkal (District), Tiruchengode, Tamil Nadu 637 215, India
2
Department of Biotechnology, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha Nagar, Chennai, Tamil Nadu 602105, India
global nanotechnology industry by 2020 [2]. Nanomaterials have specific behaviors and unique physicochemical properties in comparison to their bulk materials and have large surface area to volume ratio [3]. The unique properties of nanomaterials have attracted significant attentions for their pharmaceutical applications [4–8]. Different characteristics of nanomaterials such as morphology, size 3
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4
Depart
Data Loading...
