Increased HLA-G Expression in Tissue-Infiltrating Cells in Inflammatory Bowel Diseases
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ORIGINAL ARTICLE
Increased HLA‑G Expression in Tissue‑Infiltrating Cells in Inflammatory Bowel Diseases Sandro da Costa Ferreira1 · Ibrahim Abiodoun Sadissou2 · Rogério Serafim Parra3 · Marley Ribeiro Feitosa3 · Fermino Sanches Lizarte Neto4 · Daniela Pretti da Cunha Tirapelli4 · Leandra Naira Zambelli Ramalho5 · Omar Féres3 · José Joaquim Ribeiro da Rocha3 · Eduardo Antônio Donadi2 · Luiz Ernesto de Almeida Troncon1 Received: 8 May 2020 / Accepted: 11 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Background Since HLA-G is an immune checkpoint molecule and since Crohn’s disease (CD) and ulcerative colitis (UC) exhibit deregulated immune-mediated mechanisms, we aimed to evaluate intestinal HLA-G expression and soluble HLA-G (sHLA-G) levels in CD/UC patients stratified according to the CD phenotype/localization and UC extension. Methods HLA-G tissue expression was assessed by immunohistochemistry in biopsies collected from 151 patients (90 CD, 61 UC) and in surgical resection specimens (28 CD, 12 UC). Surgical material from 24 healthy controls was also assessed. Plasma sHLA-G levels (97 CD, 81 UC, and 120 controls) were evaluated using ELISA. Results HLA-G expression was similarly observed in the intestinal epithelial cells of control and CD/UC specimens. However, in biopsies, the plasma cells/lymphocytes infiltrating the lamina propria in CD/UC presented (1) increased HLA-G expression compared to controls (P
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