Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells
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(2020) 20:301
BMC Complementary Medicine and Therapies
RESEARCH ARTICLE
Open Access
Isatis root polysaccharide promotes maturation and secretory function of monocyte-derived dendritic cells Xuebing Wang1,2†, Zewen Chen1†, Tong Chen1, Xiao Li3, Shucheng Huang1, Hao Wang1, Chao Tong1,4* Fang Liu1*
and
Abstract Background: Pseudorabies virus (PRV) is an animal virus that is globally responsible for the high economic losses in the swine industry. Isatis root is a traditional Chinese medicinal herb that possesses immune-enhancing and antiviral properties. However, the molecular mechanisms underlying the effects of the active component of the isatis root polysaccharide (IRPS) extract on immature dendritic cells remain elusive. Methods: In this study, we investigated the molecular changes in primary porcine peripheral blood monocyte-derived dendritic cells (MoDCs) during PRV infection, using enzyme-linked immunosorbent assay (ELISA) and quantitative reverse transcription-polymerase chain reaction. Additionally, we studied the effect of IRPS on PRV-infected DCs. Results: The results showed that IRPS stimulated the maturation of MoDCs, induced IL-12 secretion, and downregulated IL-6 expression. Conclusions: Collectively, these results suggest that IRPS is a promising candidate for promoting maturation of DCs and enhancing their secretory potential after PRV infection. Keywords: Monocyte-derived DCs, Isatis root polysaccharide, Pseudorabies virus
Background Dendritic cells (DCs) play a critical role in linking innate and adaptive immunity by presenting foreign or malignant antigenic peptides to T lymphocytes [1, 2]. As a major immune surveillance cell, the DC expresses multiple pattern recognition receptors including Toll-like receptors (TLRs), Fc receptors, and C-type lectin receptors that recognize numerous pathogen-associated molecular patterns [3]. During virus infection and/or antigen recognition, DCs progressively mature and produce increasing amounts of cytokines, express costimulatory molecules, * Correspondence: [email protected]; [email protected] † Xuebing Wang and Zewen Chen are contributed equally to this work. 1 College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, Henan province, People’s Republic of China Full list of author information is available at the end of the article
and present antigens via the major histocompatibility complex (MHC) class I and II molecules. DCs stimulate naïve CD8+ and CD4+ T lymphocytes to differentiate into cytotoxic T cells and helper T (Th) cells, respectively, by antigen presentation and CD28 co-stimulation; additionally, they can present antigens to B lymphocytes and induce B cell proliferation and differentiation [4]. DCs also mediate immunological tolerance that leads to unresponsiveness to pathogenic antigens. Moreover, the IL-10 released from DCs regulates T cell anergy by stimulating regulatory T cell differentiation that suppresses T cell responses, including proliferation and IFN-γ production [5]. DC maturation is critical i
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