Isomeric glucose recognition using molecularly imprinted polymer hydrogels
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G6.2.1
Isomeric glucose recognition using molecularly imprinted polymer hydrogels
Paraskevi Parmpi, Linden D.V. Bolisay, and Peter Kofinas Department of Chemical Engineering, University of Maryland, College Park, MD 20742-2111, U.S.A.
ABSTRACT
The goal of this research is to produce molecular imprinted polymers (MIPs), which selectively bind glucose over other sugars. MIP hydrogels against glucose exhibited binding capacities in excess of 0.6 grams of glucose per gram of dry gel in a 100 % DI H2O glucose solution, as well as in a 50-50 % glucose-fructose solution mixture. Equilibrium binding capacities of fructose were lower than those observed with respect to glucose, indicating an isomeric preference for the binding of glucose over fructose. Although it is expected that imprinted cavities will be distorted due to the swelling of the hydrogel in water, our experiments show that even the swollen gels exhibit remarkable glucose recognition. This synthetic and characterization methodology for MIPs might thus offer exciting avenues for novel biomimetic recognition and isomeric separation techniques.
INTRODUCTION
In the past three decades many different polymeric hydrogel products have been developed. Hydrophilic polymeric networks are now found in applications including, but not limited to drug delivery, separation, human tissue mimics, liquid absorption, and even medical bandages. Current research attempts to further expand the scope of applications of polymeric hydrogels, through the synthesis, characterization and elucidation of biomimetic sugar recognition in molecularly imprinted polymer (MIP) networks for applications as glucose sensors [1-6]. The clinical relevance of the study presented in this research relates to the development of a pharmaceutical based on a polymer hydrogel MIP, which could aid in the treatment of type II diabetes, a disease which affects over 14 million people in the United States alone. Applying the glucose MIP to a pharmaceutical or a food additive could contribute to the dietary freedom of those who suffer from type II diabetes. By ingesting these materials, the glucose would be absorbed by the hydrogels in the intestine, thereby reducing the amount of sugar actually introduced into the blood stream, and prevent elevation of blood sugar levels after meals. The imprinting strategy of this research involves a polymer gel imprinted against glucose, using a sugar analogue, glucose phosphate monosodium or monobarium salt (GPS) as the template. A novel characteristic of the imprinting technique employed in this research is the choice of the phosphate salt of the glucose, rather than pure unmodified glucose, which results in enhanced affinity and binding of the template to the primary amine groups of the polymer, and thus improved specificity for the sugar.
G6.2.2
While methods of template fixation vary among research groups, the large majority of molecular imprinting studies to date have concentrated on synthesizing imprinted polymers from a monomer, rather than crosslinking an existi
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