Larvicidal potential of different chromatographic fractions of the n-hexane extract of Artemisia scoparia against the ve
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Larvicidal potential of different chromatographic fractions of the n-hexane extract of Artemisia scoparia against the vector mosquito Culex quinquefasciatus Mirza Gul 1 & Muhammad Zahid 1 & Hazrat Ali 2 Received: 22 September 2019 / Revised: 9 August 2020 / Accepted: 14 September 2020 # African Association of Insect Scientists 2020
Abstract The present study aims to explore the larvicidal activity of n-hexane extract isolated fractions from Artemisia scoparia against third instar larvae of Culex quinquefasciatus mosquito. Twelve fractions, F1 to F12 were separated through column chromatography by using different eluent solvents such as hexane, hexane-chloroform, chloroform and chloroform-methanol, in the order of increasing polarity. Larval bioassay was done by using WHO standard guideline. Larvicidal potential of the separated fractions was evaluated at tested concentrations of 30, 60, 90 and 120 ppm. Mortality rate was scored 24 h post exposure. The fractions F3 and F4 were found more active, exhibiting LC50 values of 28.66 and 22.37 ppm and LC90 values of 53.59 and 53.16 ppm respectively. The larvicidal activity of different fractions was compared by using ANOVA analysis. Keywords Artemisia scoparia . Culex quinquefasciatus . Fractions . Larvicidal activity
Introduction It is well known that synthetic chemical insecticides have a great environmental impact. Besides their environmental impact, the development of mosquito resistance to commonly used synthetic insecticides has created the need for developing alternative control strategies. Mosquitoes transmit several diseases to humans such as dengue fever, yellow fever, malaria, filariasis and West Nile virus etc. Therefore, mosquito is the most notorious group of insects in terms of public health importance. These diseases are possibly of public health importance in those regions of the world where climate conditions most likely favour the growth and development of mosquito populations just because of their warm and moist
* Mirza Gul [email protected] * Hazrat Ali [email protected]; [email protected] 1
Department of Zoology, Islamia College Peshawar, Peshawar, Khyber Pakhtunkhwa, Pakistan
2
Environmental Chemistry, Ecotoxicology and Applied Ecology Laboratory, Environmental Chemistry Section, Department of Chemistry, University of Malakand, Chakdara, Dir Lower, Khyber Pakhtunkhwa 18800, Pakistan
environmental conditions such as tropical and sub-tropical regions, causing millions of deaths per year. There exist close relationships among land use, climate variability and parasitic diseases associated to human population (Patz et al. 2000). Therefore, preserving intact ecosystems and their endemic biodiversity (Keesing et al. 2010) and examining the impact of climate change which significantly affect the ecology of disease vector on human disease occurrence and distribution is important (Hopp and Foley 2001) and should generally reduce the prevalence of such infectious diseases. As a vector of lymphatic filariasis and West Nile
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