LncRNA H19 Inhibits the Progression of Sepsis-Induced Myocardial Injury via Regulation of the miR-93-5p/SORBS2 Axis
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ORIGINAL ARTICLE
LncRNA H19 Inhibits the Progression of Sepsis-Induced Myocardial Injury via Regulation of the miR-93-5p/SORBS2 Axis Bin Shan,1 Jia-Yan Li,1 Ya-Jiang Liu,1 Xiao-Bin Tang,1 Zheng Zhou,1 and Liang-Xian Luo
1,2
Received May 12, 2020; accepted September 8, 2020
Sepsis is an infectious disease that seriously endangers human health. It usually leads to myocardial injury which seriously endangers to the health of human beings. H19 has been confirmed to play key roles in various diseases, including sepsis. However, its function in the progression of sepsis-induced myocardial injury remains largely unknown. H9C2 cells were treated with lipopolysaccharide (LPS) to mimic sepsis-induced myocardial injury in vitro. Cell proliferation and apoptosis were detected by MTT assay and flow cytometry, respectively. In addition, gene and protein expression levels in H9C2 cells were measured by quantitative real-time PCR (qRT-PCR) and Western blotting. The levels of inflammatory cytokines in H9C2 cell supernatants were tested by ELISA. JC-1 staining was performed to observe the mitochondrial membrane potential level in H9C2 cells. H19 and SORBS2 were downregulated in H9C2 cells following LPS treatment, while miR-93-5p was upregulated. Moreover, LPS-induced cell growth inhibition and mitochondrial damage were significantly reversed by overexpression of H19. In addition, H19 upregulation notably suppressed LPS-induced inflammatory responses in H9C2 cells. Moreover, H19 sponged miR-93-5p to promote SORBS2 expression. Overall, H19 suppressed sepsis-induced myocardial injury via regulation of the miR-93-5p/SORBS2 axis. H19 attenuated the development of sepsis-induced myocardial injury in vitro via modulation of the miR-93-5p/SORBS2 axis. Thus, H19 could serve as a potential target for the treatment of sepsis-induced myocardial injury.
Abstract—
KEY WORDS: LncRNA H19; Sepsis-induced myocardial injury; miR-93-5p; SORBS2.
INTRODUCTION 1
Department of Critical Care Medicine, The First People’s Hospital of Chenzhou, No. 8, Qinnian Avenue Road, Chenzhou, 423000, Hunan Province, People’s Republic of China 2 To whom correspondence should be addressed at Department of Critical Care Medicine, The First People’s Hospital of Chenzhou, No. 8, Qinnian Avenue Road, Chenzhou, 423000, Hunan Province, People’s Republic of China. E-mail: [email protected]
Sepsis has been considered one of the hidden public health diseases [1]. In the USA, the hospitalization of sepsis patients has dramatically increased for every 100,000 people [2]. Patients who survive sepsis suffer high
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Shan, Li, Liu, Tang, Zhou, and Luo incidence of cognitive impairment [3] and a high risk of death in the following 5 years compared to non-sepsis patients [4, 5]. Based on observations by the Centers for Disease Control and Prevention, in the twenty-first century, over 14 billion dollars were spent on sepsis in North America, and the high costs for treating this diseas
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