LncRNA SNHG14 regulates the DDP-resistance of non-small cell lung cancer cell through miR-133a/HOXB13 pathway
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RESEARCH ARTICLE
Open Access
LncRNA SNHG14 regulates the DDPresistance of non-small cell lung cancer cell through miR-133a/HOXB13 pathway Li Xu1†, Yan Xu1†, Min Yang2, Jia Li1, Fang Xu1 and Bo-Lin Chen1*
Abstract Background: Recently, long non-coding RNAs (lncRNAs) have been reported to be involved in regulating chemoresistance of NSCLC, however, the role of lncRNA SNHG14 in the DDP-resistance of NSCLC remains unexplored. Methods: Relative expression of SNHG14, HOXB13 and miR-133a in DDP-resistant A549 (A549/DDP) cell and its parental cell A549 were measured using qRT-PCR. Cell proliferation viability of indicated A549/DDP cell was estimated via CCK-8 and colony formation experiments. Cell cycle and apoptosis were analyzed through flow cytometry. Expression of apoptosis-related protein and HOXB13 were detected via western blot. The interaction among SNHG14, HOXB13 and miR-133a was predicted by bioinformatics and validated by dual-luciferase reporter assay. Results: LncRNA SNHG14 and HOXB13 were upregulated while miR-133a was downregulated in A549/DDP cell line compared to A549 cell line. SNHG14 knockdown or miR-133a overexpression was demonstrated to increase the DDPsensitivity of A549/DDP cells. SNHG14 was revealed to compete with HOXB13 for miR-133a binding in A549/DDP cells. Inhibition of miR-133a in A549 cells could reverse the promotive effects of SNHG14 knockdown on DDP-sensitivity, as well as the inhibitory effects on HOXB13 expression. HOXB13 overexpression was revealed to abolish the enhanced effects of miR-133a on the sensitivity of A549/DDP cell to DDP. Conclusion: Our findings demonstrated that SNHG14 was involved in the development of DDP-resistance of A549/ DDP cells through miR-133a/HOXB13 axis, which may present a path to novel therapeutic stratagems for DDP resistance of NSCLC. Keywords: NSCLC, LncRNA SNHG14, MiR-133a, HOXB13; DDP-resistance
Highlights 1. LncRNA SNHG14 knockdown and miR-133a overexpression increased the DDP-sensitivity of A549/ DDP cell; 2. LncRNA SNHG14 targeted and inhibited miR-133a; * Correspondence: [email protected] † Li Xu and Yan Xu are co-first authors. 1 Thoracic Medicine Department 2, The Affiliated Cancer Hospital of Xiangya School of Medicine and Hunan Cancer Hospital, Central South University, No. 283, Tongzipo Road, Yuelu District, Changsha 410013, Hunan Province, PR China Full list of author information is available at the end of the article
3. LncRNA SNHG14 regulates the DDP-resistance of non-small cell lung cancer cell through miR-133a/ HOXB13 pathway
Background Lung cancer is one of the most common malignancies in the world that responsible for a considerable proportion cancer death [1]. The high mortality of lung cancer is largely due to the common diagnosis at advanced stages that impedes the curative treatment [2]. Without effective measures for early diagnosis where the tumors are still operable, and the lack of therapeutic options when
© The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International
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