Loss of Fat with Increased Adipose Triglyceride Lipase-Mediated Lipolysis in Adipose Tissue During Laying Stages in Quai
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ORIGINAL ARTICLE
Loss of Fat with Increased Adipose Triglyceride Lipase-Mediated Lipolysis in Adipose Tissue During Laying Stages in Quail Shujin Yang • Yeunsu Suh • Young Min Choi Sangsu Shin • Jae Yong Han • Kichoon Lee
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Received: 30 September 2012 / Accepted: 6 November 2012 / Published online: 21 November 2012 Ó AOCS 2012
Abstract The goal of the current study was to investigate regulation of key genes involved in lipid metabolism in adipose and liver to relate lipolytic and lipogenic capacities with physiological changes at the pre-laying, onset of laying, and actively laying stages of quail. Followed by a 50 % increase from pre-laying to onset of laying, adipose to body weight ratio was significantly reduced by 60 % from the onset of laying to the actively laying stage (P \ 0.05), mainly resulting from the significantly increased adipocyte size from the pre-laying stage to the onset of laying and reduction of adipocyte size from the onset of laying to the actively laying stage (P \ 0.05). In the adipose tissue of actively laying quail, increased protein expression and phosphorylation of adipose triglyceride lipase (ATGL) together with an elevated mRNA expression of comparative gene identification-58, an activator of ATGL, contributes to increased lipolytic activity, as proved by increased amounts of plasma non-esterified fatty acid (P \ 0.05). In addition, decreased mRNA expression of fatty acid transport protein in the actively laying quail could contribute to the adipocyte hypotrophy (P \ 0.05).
S. Yang Y. Suh Y. M. Choi S. Shin K. Lee Department of Animal Sciences, The Ohio State University, 2029 Fyffe Ct, Columbus, OH 43210, USA
In the liver, relative mRNA expression of apo-very low density lipoprotein (VLDL)-II increased significantly from the pre-laying to actively laying stages (P \ 0.05), indicating increased apoVLDL-II actively facilitated VLDL secretion in the actively laying quail. These results suggest that the laying birds undergo active lipolysis in the adipocyte, and increase VLDL secretion from the liver in order to secure a lipid supply for yolk maturation. Keywords Adipose tissue Adipose triglyceride lipase Lipid metabolism Lipolysis Egg laying Quail Abbreviations ACC Acetyl-CoA carboxylase AP Alkaline phosphatase ATGL Adipose triglyceride lipase CGI-58 Comparative gene identification-58 CSA Cross sectional area FATP1 Fatty acid transporter protein 1 FAS Fatty acid synthase GPAT Glycerol-3-phosphate O-acyltransferase LPL Lipoprotein lipase NEFA Non-esterified fatty acids RPS13 Ribosomal protein 13 TAG Triacylglycerol VLDL Very low density lipoprotein(s)
J. Y. Han WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-921, Korea
Introduction K. Lee (&) The Ohio State University Interdisciplinary Human Nutrition Program, The Ohio State University, 2029 Fyffe Ct, Columbus, OH 43210, USA e-mail: [email protected]
Yolk lipid content is critical in avian species, as it not only comprises nearly 60 % o
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