Mechanical Stimulation and Diameter of Fiber Scaffolds Affect the Differentiation of Rabbit Annulus Fibrous Stem Cells
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Online ISSN 2212-5469
ORIGINAL ARTICLE
Mechanical Stimulation and Diameter of Fiber Scaffolds Affect the Differentiation of Rabbit Annulus Fibrous Stem Cells Pinghui Zhou1,2 • Bangguo Wei1,2 • Jingjing Guan1 • Yu Chen2,3,4 • Yansong Zhu3 • Yuchen Ye1 • Yue Meng1 • Jianzhong Guan1,2 • Yingji Mao1,2,3
Received: 25 August 2020 / Revised: 12 September 2020 / Accepted: 20 September 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract BACKGROUND: Degeneration of the annulus fibrosus (AF), an important structure of the intervertebral disc, is one of the main causes of degenerative disc disease. Fabrication of scaffolds replicating the stratified microstructure of the AF is critical for the successful regeneration of AF. METHODS: In this study, we cultured rabbit AF-derived stem cells (AFSCs) using fabricated electrospun fibrous poly-Llactic acid scaffolds with different diameters. We applied cyclic tensile strain (CTS) on the scaffolds to regulate the differentiation of AFSCs into specific cell types that resided at the inner, middle, and outer zones of the AF. RESULTS: We found that the morphologies of AFSCs on the smaller-fiber-diameter scaffolds were nearly round, whereas spindle-like cells morphologies were observed on large-diameter scaffolds. CTS enhanced these phenomena and made the cells slender. The expression levels of collagen-I in cells increased as a function of the fiber diameter, whereas collagen-II and aggrecan exhibited opposite trends. Moreover, the application of CTS upregulated the gene expressions of collagen-I, collagen-II, and aggrecan. CONCLUSION: Overlaying the scaffolds with different CTS-stimulated cells could eventually lead to engineered AF tissues with hierarchical structures that approximated the native AF tissue. Thus, the proposed methodologies could be potentially applied for AF regeneration. Keywords Intervertebral disc degeneration Cyclic tensile strain Poly-L-lactic acid scaffold Annulus fibrosus-derived stem cells Differentiation
Pinghui Zhou and Bangguo Wei have contributed equally to this work. & Jianzhong Guan [email protected] & Yingji Mao [email protected]; [email protected] 1
Department of Orthopedics, First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China
2
Anhui Province Key Laboratory of Tissue Transplantation, Bengbu Medical College, Bengbu 233030, China
3
School of Life Sciences, Bengbu Medical College, Bengbu 233030, China
4
Department of Plastic Surgery, the First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China
1 Introduction Discogenic low back pain (LBP) is one of the most prevalent musculoskeletal conditions. It affects 85% of the adult population at some point during their lifetime and invariably leads to a considerable socioeconomic burden [1, 2]. Approximately 10% of patients are chronically disabled as a result of this condition. As such, LBP decreases the productivity and living quality, while the estimated healthcare expenditures in the United States exceed $85 billion
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