MicroRNA-9 expression is a prognostic biomarker in patients with osteosarcoma
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WORLD JOURNAL OF SURGICAL ONCOLOGY
RESEARCH
Open Access
MicroRNA-9 expression is a prognostic biomarker in patients with osteosarcoma Shi-hong Xu1, Yong-liang Yang1, Shu-mei Han2 and Zong-hui Wu3*
Abstract Background: The purpose of the present study was to examine the expression levels of microRNA-9 (miR-9) in osteosarcoma tissues and normal bone tissues, and investigate the relationships between miR-9 expression, clinicopathological features and the prognosis of patients with osteosarcoma. Methods: The expression levels of miR-9 in osteosarcoma tissues and corresponding non-cancerous tissues were detected using a real-time quantitative assay. Differences in patient survival were determined using the Kaplan–Meier method and a log-rank test. A Cox proportional hazards regression analysis was used for univariate and multivariate analyses of prognostic values. Results: Compared to non-cancerous bone tissues, the expression levels of miR-9 in osteosarcoma tissues were significantly elevated (P < 0.001). We found that the expression level of miR-9 was significantly associated with tumor size (P = 0.011), clinical stage (P = 0.009) and distant metastasis (P < 0.001). The Kaplan–Meier curve showed that patients with low miR-9 expression survived significantly longer than patients with high miR-9 expression (P = 0.0017). Multivariate analysis suggested that miR-9 expression level (P = 0.002) is an independent prognostic factors for overall survival. Conclusions: The findings of our study suggest that increased miR-9 expression has a strong correlation with the aggressive progression of osteosarcoma and its overexpression is a statistically significant risk factor affecting overall survival, suggesting that increased miR-9 expression could be a valuable marker of tumor progression and for prognosis of osteosarcoma. Keywords: Osteosarcoma, MicroRNA-9, Prognosis
Background Osteosarcoma is the most common primary malignancy in children and adolescents, accounting for 20 to 35% of all malignant primary bone tumors [1-3]. Although considerable advances in tumor excision technology, adjuvant chemotherapy and radiotherapy have significantly increased the survival rate of patients with osteosarcoma, the survival of osteosarcoma patients with lung metastasis and at an advanced clinical stage is quite poor [4]. A greater understanding of osteosarcoma is essential for developing novel approaches to increase survival rates. To our disappointment, despite the various efforts of basic research and clinical practice, the molecular * Correspondence: [email protected] 3 Department of ultrasound diagnosis, Shandong Medical Imaging Research Institute, Shandong University, 324 Jingwu Road, Jinan, Shandong Province 250021, China Full list of author information is available at the end of the article
genetic mechanisms and the biology involved in osteosarcoma remain poorly understood [5]. MicroRNAs (miRNAs) are small non-coding RNA molecules that play an important role in the regulation of mRNA expression. miRNAs are known to be involve
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