Molecular Detection of Human Adenovirus and Rotavirus in Feces of White-Eared Opossums
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Ó 2020 EcoHealth Alliance
Original Contribution
Molecular Detection of Human Adenovirus and Rotavirus in Feces of White-Eared Opossums Paulo Q. Menezes,1 Thassiane T. Silva,1 Fernanda B. Simas,1 Rodrigo K. Brauner,1 Paulo Bandarra,1 Meriane Demoliner,2 Ana K. A. Eisen,2 Paula Rodrigues,2 Fernando R. Spilki,2 Geferson Fischer,3 and Silvia de O. Hu¨bner 3 1
Wildlife Rehabilitation Nucleus and Wild Animal Screening Center, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil Laborato´rio de Microbiologia Molecular, Instituto de Cieˆncias da Sau´de, Universidade Feevale, Novo Hamburgo, Rio Grande do Sul, Brazil 3 Departamento de Medicina Veterina´ria Preventiva, Faculdade de Veterina´ria, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul CEP 96160-000, Brazil 2
Abstract: The white-eared opossums (Didelphis albiventris) is the largest Brazilian marsupial and a great example of animal synanthropy. Considering the high potential as a carrier of viruses originating from environmental contamination, the presence of Human adenovirus (AdV) and rotavirus was investigated in the feces of rescued white-eared opossums, which were in the process of rehabilitation. The feces of 49 animals were initially investigated by immunochromatography, with three samples positive for AdV and one sample positive for rotavirus. When submitted to PCR and nested PCR, the samples of six animals were positive for AdV and three animals were positive for group A rotavirus. Two positive samples in the immunochromatographic assay were not confirmed by PCR. After sequencing and phylogenetic analysis of AdV samples, all were identified within the genus Mastadenovirus, one being HAdV-C, four HAdV-E, and one being similar to a Mastadenovirus found in primates. This is the first report of molecular confirmation of human adenovirus and rotavirus in white-eared opossums. These data could be important of anticipation some emerging diseases and their effects on ecosystems health. Keywords: Didelphidae, Environmental virology, Zoonosis, Mastadenovirus, Rotavirus A, Brazil
INTRODUCTION Some wild species have adapted to live with humans and have settled in urbanized areas, adapting to such conditions despite man’s will. These species are classified as synanthropic (Daszak et al. 2000). The genus Didelphis is the example of synanthropic animal, being represented in
Correspondence to: Silvia de O. Hu¨bner, e-mail: [email protected]
Brazil by three species: the black-eared opossum (Didelphis aurita), the common opossum (Didelphis marsupialis), and the white-eared opossum (Didelphis albiventris) (Gonc¸alves et al. 2009). The white-eared opossum (Lund 1840) is the largest of the Brazilian marsupials, inhabiting gallery forests as well as open areas, such as fields. Opossums act as effective seed dispersers, and the species is classified as generalist in relation to their diet and habitat selection. These facts enable them to obtain success in well-wooded rural and urban areas (Lessa and Geise 2010). There are indications that the
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