Multicenter fresh frozen tissue sampling in colorectal cancer: does the quality meet the standards for state of the art
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Multicenter fresh frozen tissue sampling in colorectal cancer: does the quality meet the standards for state of the art biomarker research? Z. S. Lalmahomed . R. R. J. Coebergh van den Braak . M. H. A. Oomen . S. P. Arshad . P. H. J. Riegman . J. N. M. IJzermans . on behalf of the MATCH study working group
Received: 20 October 2016 / Accepted: 23 February 2017 Ó The Author(s) 2017. This article is published with open access at Springerlink.com
Abstract The growing interest in the molecular subclassification of colorectal cancers is increasingly facilitated by large multicenter biobanking initiatives. The quality of tissue sampling is pivotal for successful translational research. This study shows the quality of fresh frozen tissue sampling within a multicenter cohort study for colorectal cancer (CRC) patients. Each of the seven participating hospitals randomly contributed ten tissue samples, which were collected following Standard Operating Procedures (SOP) using established techniques. To indicate if the amount of intact RNA is sufficient for molecular discovery research and prove SOP compliance, the RNA integrity number (RIN) was determined. Samples with a RIN \ 6 were measured a second time and when consistently low a third time. The highest RIN was used for further analysis. 91% of the tissue samples had a RIN C 6 (91%). The remaining six samples had a RIN between 5 and 6 (4.5%) or lower than 5 (4.5%). The median overall RIN was 7.3 (range 2.9–9.0). The median RIN of samples in the university hospital homing the biobank was 7.7 and the median RIN for Z. S. Lalmahomed R. R. J. Coebergh van den Braak (&) J. N. M. IJzermans Department of Surgery, Erasmus MC Medical Center, PO Box 2040, 3000 CA Rotterdam, The Netherlands e-mail: [email protected] M. H. A. Oomen S. P. Arshad P. H. J. Riegman Department of Pathology, Erasmus MC Medical Center, Rotterdam, The Netherlands
the teaching hospitals was 7.3, ranging from 6.5 to 7.8. No differences were found in the outcome of different hospitals (p = 0.39). This study shows that the collection of high quality fresh frozen samples of colorectal cancers is feasible in a multicenter design with complete SOP adherence. Thus, using basic sampling techniques large patient cohorts can be organized for predictive and prognostic (bio)marker research for CRC. Keywords Colorectal cancer Biobank Tissue quality RNA integrity number
Introduction Colorectal cancer (CRC) is the second most common malignancy in the Western World (DeSantis et al. 2014). As in all cancer research, there is a strong trend towards molecular subclassification of CRC (Guinney et al. 2015). The studies conducted to identify these molecular and clinically relevant markers demand large numbers of patients with accurate long-term clinical data combined with high quality tissue samples to be able to use state of the art techniques (Riegman et al. 2007, 2008). Subsequently, the standard enclosed formalin-fixed paraffin-embedded tissue can be used to develop assays for daily clinical pract
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