Nanoemulsion Loaded with Volatile Oil from Piper alatipetiolatum as an Alternative Agent in the Control of Aedes aegypti
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ORIGINAL ARTICLE
Nanoemulsion Loaded with Volatile Oil from Piper alatipetiolatum as an Alternative Agent in the Control of Aedes aegypti André C. de Oliveira 1,2 & Ingrity S. C. Sá 1 & Rochelly S. Mesquita 1 & Brenner L. Pereira 3 & Leandro A. Pocrifka 3 & Tatiane P. de Souza 4 & Jesus R Rodriguez Amado 4 & Sidney G. Azevedo 5 & Edgar A. Sanches 5 & Sergio M. Nunomura 6 & Rosemary A. Roque 2 & Wanderli P. Tadei 2 & Rita C. S. Nunomura 1 Received: 12 May 2020 / Accepted: 27 August 2020 # Sociedade Brasileira de Farmacognosia 2020
Abstract Diseases transmitted by the mosquito Aedes aegypti, Culicidae, are a serious public health problem worldwide, especially because of the increasing resistance to synthetic insecticides. Volatile oils are botanicals with interesting multi-target properties, but with reduced applications due to its volatility and water solubility. For this reason, nanoemulsions containing volatile oils are an important alternative for the mosquito control. The volatile oil of leaves Piper alatipetiolatum Yunck., Piperaceae, was characterized mostly by sesquiterpenes, such as ishwarone (78.6%), ishwarol B (8.2%), β-elemene (6.9%), selin-11-en-4α-ol (2.9%), and ishwarane (2.4%). The volatile oil showed lower ovicidal activity (19 to 100%), larvicidal activity (LC50 33.74 ppm), and pupicidal activity (LC50 65.06 ppm) as compared with its nanoemulsion that presented higher ovicidal activity (47.7 to 100%), larvicidal activity (LC50 6.37 ppm), and pupicidal activity (LC50 9.33 ppm) against Ae. aegypti. The volatile oil nanoemulsion was characterized with approximately spherical morphology, with an average size of 316 ± 8 nm, PDI of 0.44 ± 0.01, zeta potential of − 8.5 ± 0.1 mV, and pH of 5.3 ± 0.1. These results indicated that the nanoemulsion containing the encapsulated volatile oil was effective to interrupt the development of immature forms of Ae. aegypti and represents an efficient alternative tool to control this vector, contributing to vector control of dengue and other related diseases. Keywords Ishwarane . Sesquiterpenes . Nanoemulsion . Insecticide . GC-FID . Two-column
Introduction In many countries of the Asian, African, and American continents, the mosquito Aedes aegypti Linnaeus, 1762 is the main vector of dengue, zika, urban yellow fever, and chikungunya (Aguirre-Obando et al. 2016; Ahamed et al. 2017). From
January to April 2019, the World Health Organization registered 451,685 cases of dengue, 24,120 cases of chikungunya, and 3085 cases of zika in Brazil (WHO 2018). Recently, the excessive use of synthetic insecticides for vector control (Aguirre-Obando et al. 2016; Carmo et al. 2017) resulted in the appearance of resistance. Besides
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s43450-020-00092-8) contains supplementary material, which is available to authorized users. * Rita C. S. Nunomura [email protected] 1
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Laboratório de Tecnologia Farmacêutica Inovação e Desenvolvimento, Faculdade de Ciências Farmacêuticas, Universidade
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