Ni and TiO 2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts
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RESEARCH ARTICLE
Ni and TiO2 nanoparticles cause adhesion and cytoskeletal changes in human osteoblasts Michal Štefančík 1 & Lucie Válková 1 & Jana Veverková 1 & Jan Balvan 1,2 & Tomáš Vičar 2 & Petr Babula 2 & Josef Mašek 3 & Pavel Kulich 4 & Monika Pávková Goldbergová 1 Received: 28 May 2020 / Accepted: 16 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Titanium-based alloys have established a crucial role in implantology. As material deteriorates overtime, nanoparticles of TiO2 and Ni are released. This study is focused on the impact of TiO2 and Ni nanoparticles with size of 100 nm on cytoskeletal and adhesive changes in human physiological and osteoarthritic osteoblasts. The impact of nanoparticles with concentration of 1.5 ng/mL on actin and tubulin expression and gene expression of FAK and ICAM-1 was studied. The cell size and actin expression of physiological osteoblasts decreased in presence of Ni nanoparticles, while TiO2 nanoparticles caused increase in cell size and actin expression. Both cell lines expressed more FAK as a response to TiO2 nanoparticles. ICAM-1 gene was overexpressed in both cell lines as a reaction to both types of nanoparticles. The presented study shows a crucial role of Ni and TiO2 nanoparticles in human osteoblast cytoskeletal and adhesive changes, especially connected with the osteoarthritic cells. Keywords Ni nanoparticles . TiO2 nanoparticles . Osteoblasts . Cytoskeleton . Adhesion . Bone
Background Alloys based on titanium (nickel-titanium, titanium) have established an important role as biomaterials in medicine over
the years, especially in the fields of vascular stent and bone replacement materials. As these materials interact with the human body, there are two forms of interactions described — the leakage of nickel and titanium ions
Highlights • Osteoblasts incorporate both single nanoparticles and TiO2 nanoparticle clusters. • Ni nanoparticles decrease size of physiological osteoblasts and actin expression. • TiO2 nanoparticles elongate physiological osteoblasts and increase actin expression. • Tubulin expression is not affected by Ni and TiO2 nanoparticles. • Osteoblasts respond to Ni and TiO2 nanoparticles via ICAM-1 gene overexpression. Responsible Editor: Lotfi Aleya Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11356-020-10908-8) contains supplementary material, which is available to authorized users. * Monika Pávková Goldbergová [email protected]
3
Department of Pharmacology and Immunotherapy, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
1
Institute of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
4
Department of Chemistry and Toxicology, Veterinary Research Institute, Hudcova 296/70, 621 00 Brno, Czech Republic
2
Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
Environ Sci Pollut Res
(Ševčíková et al. 2018; Høl
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