Nucleic Acid Hybridization in the Study of Cell Differentiation
The informational content of cells is encoded in the nucleotide sequences of their DNA. The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcript
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3 Advisory Board
R. Brown, Edinburgh · F. Duspiva, Heidelberg · H. Eagle, Bronx · M. Feldman, Rehovoth P. R. Gross, Cambridge · H. Ha"is, Oxford · H. Holtzer, Philadelphia · W. A. Jensen, Berkeley· R. ]. Neff, Nashville · D. H. Northcote, Cambridge · C. Pe/ling, Tiibingen W. Sachsenmaier, lnn.rbruck · H. A. Schneiderman, Irvine · E. Schnepf, Heidelberg H. G. Schweiger, Wilhelmshaven ·G. L. Stebbins, Davis· C. Stern, Berkeley· H. Tiedemann, Berlin· W. Trager, New York· R. Weber, Bern· R. Wol/giehn, Halle· T.Yamada, Oak Ridge
Editors
W. Beermann, Tiibingen · ]. Reinert, Berlin · H. Ursprung, Zurich
Nucleic Acid Hybridization in the Study of Cell Differentiation Edited by H. Ursprung, Zurich
With contributions of M. Birnstiel, Edinburgh · I. R. Brown, Calgary R. B. Church, Calgary · R. C. C. Huang, Baltimore· K. H. Kim, West Lafayette · I. Purdom, Glasgow · M. M. Smith, Baltimore D. M. Steffensen, Urbana ·H. Tobler, Ziirich · R. Williamson, Edinburgh · D. E. Wimber, Eugene
With 29 Figures
Springer-Verlag Berlin Heidelberg GmbH 1972
ISBN 978-3-662-22245-4 ISBN 978-3-540-37149-6 (eBook) DOI 10.1007/978-3-540-37149-6
This werk is subject to copyright. All rights are reserved, whether the whole or patt of the material is concemed, specifically those of translation, reprinting, re-use of illustrations, broadcasring, reproduction by photocopying machine or similar means, and storage in data banks. Under § 54 of the German Copyright Law, where copies are made for other than private use, a fee is payabie to the publisher, the amount of the fee to be determined by agreement with the publisher. The use of general descriptive names, trade names, trade marks etc. in this publication, even if the formet are not especially identified, is nottobe taken as a sign that such names, as understood by the TradeMarksand Merchandise Marks Act, may accordingly be used freely by anyone.
© by Springer-Verlag Berlin Beideiberg 1972. Originally published by Springer-Verlag Berlin Heidelberg New York in 1972 Softcoverreprint ofthe hardcoverIst edition 1972 Library of CongressCatalog CardNumber 70-188705.
Preface The informational content of cells is encoded in the nucleotide sequences of their DNA. The restrictions on base pairing- A pairing with T (U), and G pairing with C - in nature assures the fidelity of replication of DNA in cell division, and of transcription. In the test tube, these restrictions can be exploited for ascertaining similarities and dissimilarities of nucleic acids of varying origin by measuring the kinetics of reassociation of polynucleotides to double-stranded molecules in DNADNA renaturation or RNA-DNA hybridization experiments, and by determining the thermal stability and other physical-chemical properties of the resulting hybrid molecules. This method has enormous potential for developmental biology. It offers a more direct approach to the ever-present question of the genetic identity of different cell types in an individual organism, and a more direct test of the hypothesis of differential gene function. I
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