Overexpression of MaTPD1A impairs fruit and pollen development by modulating some regulators in Musa itinerans
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RESEARCH ARTICLE
Open Access
Overexpression of MaTPD1A impairs fruit and pollen development by modulating some regulators in Musa itinerans Chunhua Hu1,2,3, Ou Sheng1,2,3, Tao Dong1,2,3, Qiaosong Yang1,2,3, Tongxin Dou1,2,3, Chunyu Li1,2,3, Weidi He1,2,3, Huijun Gao1,2,3, Ganjun Yi1,2,3, Guiming Deng1,2,3* and Fangcheng Bi1,2,3*
Abstract Background: Pollen formation and development is important for crop fertility and is a key factor for hybrid development. Previous reports have indicated that Arabidopsis thaliana TAPETUM DETERMINANT1 (AtTPD1) and its rice (Oryza sativa) homolog, OsTPD1-like (OsTDL1A), are required for cell specialization and greatly affect pollen formation and development. Little is known about the role of the TPD1 homolog in banana pollen development. Results: Here, we report the identification and characterization of TPD1 homologs in diploid banana (Musa itinerans) and examine their role in pollen development by overexpressing the closest homolog, MaTPD1A. MaTPD1A exhibits high expression in stamen and localizes in the plasma membrane. MaTPD1A-overexpressing plants produce no pollen grains and smaller and seedless fruit compared to wild-type plants. Transcriptome analysis showed that in plant hormone, starch and sucrose metabolism, and linolenic acid metabolism-related pathways were affected by overexpression of MaTPD1A, and the expression of several key regulators, such as PTC1 and MYB80, which are known to affect anther development, is affected in MaTPD1A-overexpressing lines. Conclusions: Our results indicate that MaTPD1A plays an important role in pollen formation and fruit development in diploid banana, possibly by affecting the expression of some key regulators of pollen development. Keywords: Wild banana fruits develop, TPD1, Male sterile, Musa itinerans, Overexpression
Background Pollen development, release, and pollination are vital for successful plant sexual reproduction and genetic diversity in the world. The development of hybrid lines with genetically modified (GM) pollen might group multiple important agronomic traits together and result in great hybrid vigor. In the anther of flowering plants, pollen formation results from the differentiation and interaction of reproductive cells (microsporocytes) with somatic anther wall cells (tapetum) [1]. In Arabidopsis and * Correspondence: [email protected]; [email protected] 1 Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou, China Full list of author information is available at the end of the article
rice, an increasing number of genes that are involved in pollen development and formation have been determined through characterization of male sterile mutants in both species, and the key genes that control the process of pollen development in both species are relatively conserved [2–4]. In Arabidopsis, rice and Maize, a pair of protein-protein interactors that complex TAPETUM DETERMINANT1 (AtTPD1): EXCESS MICROSPOROCYTES1/EXTRA SPOROGENOUS CELLS (AtEMS1/EXS) in Arabidopsis [5–7], OsTDL1A:
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