Ectopic overexpression of a cotton plastidial Na + transporter GhBASS5 impairs salt tolerance in Arabidopsis via increas
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ORIGINAL ARTICLE
Ectopic overexpression of a cotton plastidial Na+ transporter GhBASS5 impairs salt tolerance in Arabidopsis via increasing Na+ loading and accumulation Thwin Myo1,2 · Baoming Tian1,2 · Qi Zhang1,2 · Shasha Niu1,2 · Zhixian Liu1,3 · Yinghui Shi3 · Gangqiang Cao1,2 · Hua Ling3 · Fang Wei1,2 · Gongyao Shi1,2 Received: 24 May 2020 / Accepted: 18 August 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Main conclusion GhBASS5 is a member of the bile acid sodium symporter (BASS) gene family from cotton and a plastid-localized Na+ transporter that negatively regulates salt tolerance of plants. Abstract Soil salinization is a major constraint on global cotton production, and Na+ is the most dominant toxic ion in salinity stress. Hence, insights into the identities and properties of transporters that catalyze Na+ movement between different tissues and within the cell compartments are vital to understand the salt-tolerant mechanisms of plants. Here, we identified the GhBASS5 gene, a member of the bile acid sodium symporter (BASS) gene family from cotton, served as a plastidic Na+ transporter. GhBASS5 encodes a membrane protein localized in the plastid envelope. It was highly expressed in cotton roots and predominantly existed in the vascular cylinder. Heterogenous expression of GhBASS5 in Arabidopsis chloroplasts promoted Na+ uptake into chloroplasts, which contributed to an increased cytoplasmic N a+ concentration. And GhBASS5+ overexpressed transgenic plants showed an increase in Na translocation from roots to shoots and an elevated Na+ content in both roots and shoots, but a dramatic decrease in the N a+ efflux from root tissues and the K+/Na+ ratio, especially under salt stress conditions. Furthermore, overexpressing GhBASS5 greatly damaged plastid functions and enhanced salt sensitivity in transgenic Arabidopsis when compared with wild-type plants under salt stress. Additionally, the salt-responsive transporter genes that regulate K+/Na+ homeostasis were dramatically expressed in GhBASS5-overexpressed lines, especially under salt stress conditions. Taken together, our results suggest that GhBASS5 is a plastid-localized Na+ transporter, and high expression of GhBASS5 impairs salt tolerance of plants via increasing Na+ transportation and accumulation at both cell and tissue levels. Keywords Gossypium hirsutum bile acid sodium symporter 5 (GhBASS5) · Plastid-localized Na+ transporter · Overexpression · Transgenic arabidopsis · Salt stress
Communicated by Dorothea Bartels. Thwin Myo and Baoming Tian are contributed equally to this article Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00425-020-03445-8) contains supplementary material, which is available to authorized users. * Fang Wei [email protected] * Gongyao Shi [email protected] 1
Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450001, Henan, China
Abbreviations BASS Bile acid sodium symporter GhBASS5 Gos
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